Rat Ryanodine Receptor 2, Cardiac (RYR2) ELISA Kit | RYR2 elisa kit

Rat Ryanodine Receptor 2, Cardiac (RYR2) ELISA Kit

Cat. Num. AAA2707610

• Gene Names: RYR2; RyR; ARVC2; ARVD2; RYR-2; VTSIP
• Reactivity: Rat
• Synonyms: Ryanodine Receptor 2; Cardiac (RYR2); Rat Ryanodine Receptor 2; Cardiac (RYR2) ELISA Kit; RYR-2; ARVD2; ARVC2; VTSIP; Arrhythmogenic Right Ventricular Dysplasia 2; Cardiac muscle ryanodine receptor-calcium release channel; RYR2 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Ryanodine Receptor 2, Cardiac (RYR2).; No significant cross-reactivity or interference between Ryanodine Receptor 2, Cardiac (RYR2) and analogues was observed.
• Sequence Length: 4967

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.122ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ryanodine Receptor 2, Cardiac (RYR2) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ryanodine Receptor 2, Cardiac (RYR2) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RYR2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ryanodine Receptor 2, Cardiac (RYR2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ryanodine Receptor 2, Cardiac (RYR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ryanodine Receptor 2, Cardiac (RYR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ryanodine Receptor 2, Cardiac (RYR2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 112799847
• NCBI GeneID: 6262
• NCBI GenBank Nucleotide #: NP_001026.2
• UniProt Accession #: Q92736
• NCBI Official Full Name: ryanodine receptor 2
• NCBI Official Synonym Full Names: ryanodine receptor 2
• NCBI Official Symbol: RYR2
• NCBI Official Synonym Symbols: RyR; ARVC2; ARVD2; RYR-2; VTSIP
• NCBI Protein Information: ryanodine receptor 2
• UniProt Protein Name: Ryanodine receptor 2
• Protein Family: Ryanodine receptor
• UniProt Gene Name: RYR2
• UniProt Synonym Gene Names: RYR-2; RyR2; hRYR-2
• UniProt Entry Name: RYR2_HUMAN

NCBI Description

This gene encodes a ryanodine receptor found in cardiac muscle sarcoplasmic reticulum. The encoded protein is one of the components of a calcium channel, composed of a tetramer of the ryanodine receptor proteins and a tetramer of FK506 binding protein 1B proteins, that supplies calcium to cardiac muscle. Mutations in this gene are associated with stress-induced polymorphic ventricular tachycardia and arrhythmogenic right ventricular dysplasia. [provided by RefSeq, Jul 2008]

Uniprot Description

RYR2: ryanodine receptor calcium release channel. Regulates communication between transverse-tubules and sarcoplasmic reticulum. Contraction of cardiac muscle is triggered by release of calcium ions from SR following depolarization of T-tubules. Phosphorylation by PKA dissociates FKBP12.6 from RYR2. Two splice isoforms have been described.

Protein type: Transporter, ion channel; Membrane protein, integral; Membrane protein, multi-pass; Channel, ligand-gated; Transporter

Chromosomal Location of Human Ortholog: 1q43

Cellular Component: sarcoplasmic reticulum membrane; protein complex; smooth endoplasmic reticulum; membrane; sarcoplasmic reticulum; plasma membrane; Z disc

Molecular Function: calmodulin binding; calcium-induced calcium release activity; identical protein binding; protein binding; enzyme binding; protein self-association; calcium-release channel activity; calcium channel activity; calcium ion binding; intracellular ligand-gated calcium channel activity; ryanodine-sensitive calcium-release channel activity

Biological Process: positive regulation of sequestering of calcium ion; cytosolic calcium ion homeostasis; regulation of heart rate; calcium-mediated signaling; positive regulation of heart rate; response to redox state; Wnt receptor signaling pathway through beta-catenin; release of sequestered calcium ion by sarcoplasmic reticulum into cytosol; response to caffeine; BMP signaling pathway; cellular calcium ion homeostasis; release of sequestered calcium ion into cytosol; calcium ion transport; response to hypoxia; detection of calcium ion; transmembrane transport; cardiac muscle contraction

Disease: Ventricular Tachycardia, Catecholaminergic Polymorphic, 1, With Or Without Atrial Dysfunction And/or Dilated Cardiomyopathy; Arrhythmogenic Right Ventricular Dysplasia, Familial, 2