SDS-PAGE
UBE2D4 recombinant protein
Recombinant Human UBE2D4 Protein (His tag)
Gene Names
UBE2D4; HBUCE1
Purity
> 95 % as determined by SDS-PAGE
Synonyms
UBE2D4; Recombinant Human UBE2D4 Protein (His tag); HBUCE1; UBE2D4 recombinant protein
Host
E Coli
Purity/Purification
> 95 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile 20mM Tris, 0.1M NaCl, 10% glycerol, 2mM DTT, pH 8.0
Sequence Length
147
Application Notes
The recombinant human UBE2D4 consisting of 162 amino acids and migrates as an approximately 18.5 kDa band in SDS-PAGE under reducing conditions as predicted.
Predicted N Terminal
Met
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C
SDS-PAGE
SDS-PAGE
Related Product Information for UBE2D4 recombinant protein
Background: UBE2D4 is a member of the ubiquitin-conjugating E2 family whose members perform the second step in the ubiquitination reaction. Initially identified as the main process for protein degradation, ubiquitination is believed nowadays to be crucial for a wider range of cellular processes. The outcome of the ubiquitin-conjugation reaction, and thereby the fate of the substrate, is heavily dependent on the number of ubiquitin molecules attached and how these ubiquitin molecules are inter-connected. To deal with this complexity and to allow adequate ubiquitination in time and space, a highly sophisticated conjugation machinery has been developed. In a sequential manner, ubiquitin becomes activated by an ubiquitin-activating enzyme (E1), which then transfers the ubiquitin to a group of ubiquitin-conjugating enzymes (E2s). Next, ubiquitin-loaded E2s are interacting with ubiquitin protein ligases (E3s) and ubiquitin is conjugated to substrates on recruitment by the E3. These three key enzymes are operating in a hierarchical system, wherein two E1s and 35 E2s have been found and hundreds of E3s have been identified in humans. It has been identified the UBE2D family (UBE2D1-4) as E2 partners for IDOL that support both autoubiquitination and IDOL-dependent ubiquitination of the LDLR in a cell-free system.
Description: A DNA sequence encoding the human UBE2D4 (Q9Y2X8) (Met 1-Met 1474) was expressed, with a polyhistide tag at the N-terminus.
Description: A DNA sequence encoding the human UBE2D4 (Q9Y2X8) (Met 1-Met 1474) was expressed, with a polyhistide tag at the N-terminus.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16,649 Da
NCBI Official Full Name
ubiquitin-conjugating enzyme E2 D4
NCBI Official Synonym Full Names
ubiquitin conjugating enzyme E2 D4 (putative)
NCBI Official Symbol
UBE2D4
NCBI Official Synonym Symbols
HBUCE1
NCBI Protein Information
ubiquitin-conjugating enzyme E2 D4
UniProt Protein Name
Ubiquitin-conjugating enzyme E2 D4
Protein Family
UniProt Gene Name
UBE2D4
UniProt Synonym Gene Names
UBCH5D
Uniprot Description
UBE2D4: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11' and 'Lys-48'-linked polyubiquitination. Belongs to the ubiquitin-conjugating enzyme family.
Protein type: EC 6.3.2.19; Ligase; Ubiquitin conjugating system; Ubiquitin ligase
Chromosomal Location of Human Ortholog: 7p13
Molecular Function: protein binding; ubiquitin-protein ligase activity
Biological Process: protein ubiquitination
Research Articles on UBE2D4
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Product Notes
The UBE2D4 ube2d4 (Catalog #AAA2545376) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The recombinant human UBE2D4 consisting of 162 amino acids and migrates as an approximately 18.5 kDa band in SDS-PAGE under reducing conditions as predicted. Researchers should empirically determine the suitability of the UBE2D4 ube2d4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UBE2D4, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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