Human Ryanodine Receptor 2, Cardiac (RYR2) ELISA Kit | RYR2 elisa kit

Human Ryanodine Receptor 2, Cardiac (RYR2) ELISA Kit

Cat. Num. AAA2704876

• Gene Names: RYR2; RyR; ARVC2; ARVD2; RYR-2; VTSIP
• Reactivity: Human
• Synonyms: Ryanodine Receptor 2; Cardiac (RYR2); Human Ryanodine Receptor 2; Cardiac (RYR2) ELISA Kit; RYR-2; ARVD2; ARVC2; VTSIP; Arrhythmogenic Right Ventricular Dysplasia 2; Cardiac muscle ryanodine receptor-calcium release channel; RYR2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Ryanodine Receptor 2, Cardiac (RYR2).; No significant cross-reactivity or interference between Ryanodine Receptor 2, Cardiac (RYR2) and analogues was observed.
• Sequence Length: 4,975

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.116ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ryanodine Receptor 2, Cardiac (RYR2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ryanodine Receptor 2, Cardiac (RYR2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RYR2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ryanodine Receptor 2, Cardiac (RYR2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ryanodine Receptor 2, Cardiac (RYR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ryanodine Receptor 2, Cardiac (RYR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ryanodine Receptor 2, Cardiac (RYR2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 112799847
• NCBI GeneID: 6262
• NCBI Accession #: NP_001026.2
• NCBI GenBank Nucleotide #: NM_001035.2
• UniProt Accession #: Q92736; Q15411; Q546N8; Q5T3P2
• Molecular Weight: 565,411 Da
• NCBI Official Full Name: ryanodine receptor 2
• NCBI Official Synonym Full Names: ryanodine receptor 2
• NCBI Official Symbol: RYR2
• NCBI Official Synonym Symbols: RyR; ARVC2; ARVD2; RYR-2; VTSIP
• NCBI Protein Information: ryanodine receptor 2
• UniProt Protein Name: Ryanodine receptor 2
• Protein Family: Ryanodine receptor
• UniProt Gene Name: RYR2
• UniProt Synonym Gene Names: RYR-2; RyR2; hRYR-2

NCBI Description

This gene encodes a ryanodine receptor found in cardiac muscle sarcoplasmic reticulum. The encoded protein is one of the components of a calcium channel, composed of a tetramer of the ryanodine receptor proteins and a tetramer of FK506 binding protein 1B proteins, that supplies calcium to cardiac muscle. Mutations in this gene are associated with stress-induced polymorphic ventricular tachycardia and arrhythmogenic right ventricular dysplasia. [provided by RefSeq, Jul 2008]

Uniprot Description

Calcium channel that mediates the release of Ca2+ from the sarcoplasmic reticulum into the cytoplasm and thereby plays a key role in triggering cardiac muscle contraction. Aberrant channel activation can lead to cardiac arrhythmia. In cardiac myocytes, calcium release is triggered by increased Ca2+ levels due to activation of the L-type calcium channel CACNA1C. The calcium channel activity is modulated by formation of heterotetramers with RYR3. Required for cellular calcium ion homeostasis. Required for embryonic heart development.