Typical Testing Data/Standard Curve (for reference only)
Rat Renin ELISA Kit | Renin elisa kit
Rat Renin ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: RDGLU ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-RDGLU antibody and an RDGLU-HRP conjugate. The assay sample and buffer are incubated together with RDGLU-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RDGLU concentration since RDGLU from samples and RDGLU-HRP conjugate compete for the anti-RDGLU antibody binding site. Since the number of sites is limited, as more sites are occupied by RDGLU from the sample, fewer sites are left to bind RDGLU-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RDGLU concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Renin catalyzes the first step in the activation pathway of angiotensinogen--a cascade that can result in aldosterone release,vasoconstriction, and increase in blood pressure. Renin, an aspartyl protease, cleaves angiotensinogen to form angiotensin I, which is converted to angiotensin II by angiotensin I converting enzyme, an important regulator of blood pressure and electrolyte balance. Transcript variants that encode different protein isoforms and that arise from alternative splicing and the use of alternative promoters have been described, but their full-length nature has not been determined. Mutations in this gene have been shown to cause familial hyperproreninemia. [provided by RefSeq, Jul 2008]
Uniprot Description
REN: Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. Interacts with ATP6AP2. Interaction with ATP6AP2 results in a 5-fold increased efficiency in angiotensinogen processing. Belongs to the peptidase A1 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; EC 3.4.23.15; Protease; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 1q32
Cellular Component: extracellular space; cytoplasm; plasma membrane; extracellular region
Molecular Function: peptidase activity; insulin-like growth factor receptor binding; aspartic-type endopeptidase activity; receptor binding
Biological Process: response to cAMP; hormone-mediated signaling; drinking behavior; cell maturation; renin-angiotensin regulation of aldosterone production; male gonad development; response to lipopolysaccharide; angiotensin maturation; proteolysis; cellular protein metabolic process; regulation of blood pressure; beta-amyloid metabolic process; regulation of MAPKKK cascade; mesonephros development; kidney development
Disease: Renal Tubular Dysgenesis; Hyperuricemic Nephropathy, Familial Juvenile, 2
Research Articles on Renin
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Product Notes
The Rat Renin ren (Catalog #AAA727443) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA727443 ELISA Kit recognizes Rat Renin. It is sometimes possible for the material contained within the vial of "Renin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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