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Typical Testing Data

Mouse Renin ELISA Kit | REN elisa kit

Mouse Renin ELISA Kit

Gene Names
REN; HNFJ2
Reactivity
Mouse
Synonyms
Renin; Mouse Renin ELISA Kit; REN/Renin/Angiotensinogenase/angiotensin-forming enzyme/HNFJ2/renin precursor; renal; REN elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Sequence Length
406
Samples
Serum, plasma and other biological fluids.
Assay Type
Sandwich ELISA, Double Antibody
Detection Range
93.75-6000pg/ml
Sensitivity
56.25pg/ml
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Testing Data

Typical Standard Curve

Typical Standard Curve
Related Product Information for REN elisa kit
Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse RENantibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse REN antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse REN amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofMouse RENcan be calculated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
44,726 Da
NCBI Official Full Name
renin preproprotein
NCBI Official Synonym Full Names
renin
NCBI Official Symbol
REN
NCBI Official Synonym Symbols
HNFJ2
NCBI Protein Information
renin
UniProt Protein Name
Renin
Protein Family
UniProt Gene Name
REN
UniProt Entry Name
RENI_HUMAN

NCBI Description

Renin catalyzes the first step in the activation pathway of angiotensinogen--a cascade that can result in aldosterone release,vasoconstriction, and increase in blood pressure. Renin, an aspartyl protease, cleaves angiotensinogen to form angiotensin I, which is converted to angiotensin II by angiotensin I converting enzyme, an important regulator of blood pressure and electrolyte balance. Transcript variants that encode different protein isoforms and that arise from alternative splicing and the use of alternative promoters have been described, but their full-length nature has not been determined. Mutations in this gene have been shown to cause familial hyperproreninemia. [provided by RefSeq, Jul 2008]

Uniprot Description

REN: Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. Interacts with ATP6AP2. Interaction with ATP6AP2 results in a 5-fold increased efficiency in angiotensinogen processing. Belongs to the peptidase A1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; EC 3.4.23.15; Protease

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: extracellular region; extracellular space; lysosome; plasma membrane

Molecular Function: aspartic-type endopeptidase activity; insulin-like growth factor receptor binding; peptidase activity; receptor binding

Biological Process: angiotensin maturation; beta-amyloid metabolic process; cell maturation; cellular protein metabolic process; drinking behavior; hormone-mediated signaling; kidney development; male gonad development; mesonephros development; protein catabolic process; proteolysis; regulation of blood pressure; regulation of MAPKKK cascade; renin-angiotensin regulation of aldosterone production; response to cAMP; response to lipopolysaccharide

Disease: Hyperuricemic Nephropathy, Familial Juvenile, 2; Renal Tubular Dysgenesis

Research Articles on REN

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Product Notes

The Mouse REN ren (Catalog #AAA762963) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA762963 ELISA Kit recognizes Mouse REN. It is sometimes possible for the material contained within the vial of "Renin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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