Rat proteoglycan 4 (PRG4) ELISA Kit | PRG4 elisa kit

Rat proteoglycan 4, PRG4 ELISA Kit

Cat. Num. AAA705095

• Gene Names: PRG4; MSF; SZP; CACP; HAPO; JCAP; bG174L6.2
• Reactivity: Rat
• Synonyms: proteoglycan 4 (PRG4); Rat proteoglycan 4; PRG4 ELISA Kit; Rat proteoglycan 4 (PRG4) ELISA kit; GS1-174L6.2; CACP; FLJ32635; HAPO; JCAP; MSF; SZP; bG174L6.2; OTTHUMP00000033580; articular superficial zone protein; bG174L6.2 (MSF: megakaryocyte stimulating factor) ; lubricin; megakaryocyte stimulat; PRG4 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of rat PRG4. No significant cross-reactivity or interference between rat PRG4 and analogues was observed.
• Sequence Length: 1363

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 1.56 ng/ml-100 ng/ml
• Sensitivity: < 0.39 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PRG4 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PRG4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRG4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRG4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRG4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 189181720
• NCBI GeneID: 10216
• NCBI Accession #: NP_001121180.1
• NCBI GenBank Nucleotide #: NM_001127708.1
• UniProt Accession #: Q92954; Q6DNC4; Q6DNC5; Q6ZMZ5; Q9BX49
• Molecular Weight: 151,077 Da
• NCBI Official Full Name: proteoglycan 4 isoform B
• NCBI Official Synonym Full Names: proteoglycan 4
• NCBI Official Symbol: PRG4
• NCBI Official Synonym Symbols: MSF; SZP; CACP; HAPO; JCAP; bG174L6.2
• NCBI Protein Information: proteoglycan 4; lubricin; superficial zone proteoglycan; megakaryocyte stimulating factor; megakaryocyte-stimulating factor; articular superficial zone protein; bG174L6.2 (MSF: megakaryocyte stimulating factor )
• UniProt Protein Name: Proteoglycan 4
• Protein Family: Proteoglycan
• UniProt Gene Name: PRG4
• UniProt Synonym Gene Names: MSF; SZP
• UniProt Entry Name: PRG4_HUMAN

NCBI Description

The protein encoded by this gene is a large proteoglycan specifically synthesized by chondrocytes located at the surface of articular cartilage, and also by some synovial lining cells. This protein contains both chondroitin sulfate and keratan sulfate glycosaminoglycans. It functions as a boundary lubricant at the cartilage surface and contributes to the elastic absorption and energy dissipation of synovial fluid. Mutations in this gene result in camptodactyly-arthropathy-coxa vara-pericarditis syndrome. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Plays a role in boundary lubrication within articulating joints. Prevents protein deposition onto cartilage from synovial fluid by controlling adhesion-dependent synovial growth and inhibiting the adhesion of synovial cells to the cartilage surface.2 PublicationsManual assertion based on experiment in:Ref.6