Human Proteoglycan 4 ELISA Kit | PRG4 elisa kit

Human Proteoglycan 4 ELISA Kit

Cat. Num. AAA744282

• Reactivity: Human
• Synonyms: Proteoglycan 4; Human Proteoglycan 4 ELISA Kit; PRG4 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 10-250ng/mL
• Sensitivity: 1.0ng/mL
• Intended Uses: This PRG4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PRG4. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PRG4 elisa kit

Principle of the assay: PRG4 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PRG4. Standards or samples are then added to the microtiter plate wells and PRG4 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PRG4 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for PRG4 are added to each well to “sandwich” the PRG4 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PRG4 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PRG4 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 50345548
• Molecular Weight: 146,468 Da
• NCBI Official Full Name: proteoglycan 4, partial
• UniProt Protein Name: Proteoglycan 4
• Protein Family: Proteoglycan
• UniProt Gene Name: PRG4
• UniProt Synonym Gene Names: MSF; SZP
• UniProt Entry Name: PRG4_HUMAN

Uniprot Description

PRG4: Plays a role in boundary lubrication within articulating joints. Prevents protein deposition onto cartilage from synovial fluid by controlling adhesion-dependent synovial growth and inhibiting the adhesion of synovial cells to the cartilage surface. Defects in PRG4 are the cause of camptodactyly- arthropathy-coxa vara-pericarditis syndrome (CACP); also known as Jacobs syndrome. CACP is an autosomal recessive disorder. Individuals with CACP have normal appearing joints at birth but with advancing age develop joint failure associated with noninflammatory synoviocyte hyperplasia and subintimal fibrosis of the synovial capsule. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q25-q31

Cellular Component: extracellular space

Molecular Function: scavenger receptor activity; polysaccharide binding

Biological Process: cell proliferation; receptor-mediated endocytosis; negative regulation of interleukin-6 biosynthetic process; immune response; regulation of cell proliferation

Disease: Camptodactyly-arthropathy-coxa Vara-pericarditis Syndrome