Human Proteoglycan 4 ELISA Kit | PRG4 elisa kit

Human Proteoglycan 4 ELISA Kit

Cat. Num. AAA765294

• Gene Names: PRG4; MSF; SZP; CACP; HAPO; JCAP
• Reactivity: Human
• Synonyms: Proteoglycan 4; Human Proteoglycan 4 ELISA Kit; PRG4 (Proteoglycan 4)/SZP/Superficial zone proteoglycan/Lubricin/Megakaryocyte-stimulating factor; PRG4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PRG4. No significant cross-reactivity or interference between PRG4 and analogues was observed.
• Sequence Length: 1404

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 1.25-80ng/ml
• Sensitivity: 0.75ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PRG4 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 735997426
• NCBI GeneID: 10216
• NCBI Accession #: NP_005798.3
• NCBI GenBank Nucleotide #: NM_005807.4
• Molecular Weight: 146,468 Da
• NCBI Official Full Name: proteoglycan 4 isoform A preproprotein
• NCBI Official Synonym Full Names: proteoglycan 4
• NCBI Official Symbol: PRG4
• NCBI Official Synonym Symbols: MSF; SZP; CACP; HAPO; JCAP
• NCBI Protein Information: proteoglycan 4
• UniProt Protein Name: Proteoglycan 4
• Protein Family: Proteoglycan
• UniProt Gene Name: PRG4
• UniProt Synonym Gene Names: MSF; SZP
• UniProt Entry Name: PRG4_HUMAN

NCBI Description

The protein encoded by this gene is a large proteoglycan that is synthesized by chondrocytes located at the surface of articular cartilage and by some synovial lining cells. This protein contains both chondroitin sulfate and keratan sulfate glycosaminoglycans. It functions as a boundary lubricant at the cartilage surface and contributes to the elastic absorption and energy dissipation of synovial fluid. Mutations in this gene result in camptodactyly-arthropathy-coxa vara-pericarditis syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Uniprot Description

PRG4: Plays a role in boundary lubrication within articulating joints. Prevents protein deposition onto cartilage from synovial fluid by controlling adhesion-dependent synovial growth and inhibiting the adhesion of synovial cells to the cartilage surface. Defects in PRG4 are the cause of camptodactyly- arthropathy-coxa vara-pericarditis syndrome (CACP); also known as Jacobs syndrome. CACP is an autosomal recessive disorder. Individuals with CACP have normal appearing joints at birth but with advancing age develop joint failure associated with noninflammatory synoviocyte hyperplasia and subintimal fibrosis of the synovial capsule. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q25-q31

Cellular Component: extracellular space

Molecular Function: polysaccharide binding; scavenger receptor activity

Biological Process: cell proliferation; immune response; negative regulation of interleukin-6 biosynthetic process; receptor-mediated endocytosis; regulation of cell proliferation

Disease: Camptodactyly-arthropathy-coxa Vara-pericarditis Syndrome