Rat Presenilin 1 (PSEN1) ELISA Kit | PSEN1 elisa kit

Rat Presenilin 1 (PSEN1) ELISA Kit

Cat. Num. AAA450702

• Reactivity: Rat
• Synonyms: Presenilin 1 (PSEN1); Rat Presenilin 1 (PSEN1) ELISA Kit; FAD; AD3; PS1; PSNL1; S182; Alzheimer Disease 3; Protein S182; Presenilin-1 CTF12; PSEN1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of PSEN1. No significant cross-reactivity or interference between PSEN1 and analogues was observed.
• Sequence Length: 468

• Samples: Serum, plasma, tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.061ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PSEN1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PSEN1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PSEN1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PSEN1 in rat serum, plasma, tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PSEN1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PSEN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PSEN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PSEN1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 47575859
• NCBI GeneID: 29192
• NCBI Accession #: NP_062036.2
• NCBI GenBank Nucleotide #: NM_019163.3
• UniProt Accession #: P97887; P97529
• Molecular Weight: 52,790 Da
• NCBI Official Full Name: presenilin-1
• NCBI Official Synonym Full Names: presenilin 1
• NCBI Official Symbol: Psen1
• NCBI Protein Information: presenilin-1
• UniProt Protein Name: Presenilin-1
• Protein Family: Presenilin
• UniProt Gene Name: Psen1
• UniProt Synonym Gene Names: Psnl1; PS-1; PS1-CTF12

NCBI Description

multitransmembrane protein; human homolog mutated in most cases of early onset familial Alzheimer's disease; may mediate retinal lamination and pattern formation [RGD, Feb 2006]

Uniprot Description

Probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. Stimulates cell-cell adhesion though its association with the E-cadherin/catenin complex. Under conditions of apoptosis or calcium influx, cleaves E-cadherin promoting the disassembly of the E-cadherin/catenin complex and increasing the pool of cytoplasmic beta-catenin, thus negatively regulating Wnt signaling. May also play a role in hematopoiesis ().