Hedgehog Homolog, Sonic (SHH) Recombinant Protein | SHH recombinant protein

Recombinant Hedgehog Homolog, Sonic (SHH)

Cat. Num. AAA2009508

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Hedgehog Homolog; Sonic (SHH); Recombinant Hedgehog Homolog; SHH recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-GRG FGKRQHPKKL TPLAYKQFIP NVAEKTLGAS GRYEGKITRN SERFKELTPN YNPDIIFKDE ENTGADRLMT QRCKDKLNAL AISVMNQWPG VKLRVTEGWD EDGHHSEESL HYEGRAVDIT TSDRDRSKYG MLARLAVEAG FDWVYYESKA RIHCSVKAEN SV
• Sequence Length: 437

• Applicable Applications for SHH recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Gly28~Val192 (Accession # Q63673) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 8394267
• NCBI GeneID: 29499
• NCBI Accession #: NP_058917.1
• NCBI GenBank Nucleotide #: NM_017221.1
• UniProt Accession #: Q63673
• Molecular Weight: 20.4kDa
• NCBI Official Full Name: sonic hedgehog protein
• NCBI Official Synonym Full Names: sonic hedgehog
• NCBI Official Symbol: Shh
• NCBI Protein Information: sonic hedgehog protein; sonic hedgehog homolog
• UniProt Protein Name: Sonic hedgehog protein
• Protein Family: Sonic hedgehog protein
• UniProt Gene Name: Shh
• UniProt Synonym Gene Names: Vhh-1; SHH
• UniProt Entry Name: SHH_RAT

NCBI Description

plays a role in neurogenesis; regulates proliferation of adult hippocampal neural stem cells [RGD, Feb 2006]

Uniprot Description

Function: Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction

By similarity.

Subunit structure: Interacts with HHATL/GUP1 which negatively regulates HHAT-mediated palmitoylation of the SHH N-terminus. Interacts with BOC and CDON. Interacts with HHIP

By similarity. N-product is active as a multimer. Ref.2

Subcellular location: Sonic hedgehog protein C-product: Secreted › extracellular space. Note: The C-terminal peptide diffuses from the cell. Ref.2Sonic hedgehog protein N-product: Cell membrane; Lipid-anchor. Note: The N-product either remains associated with lipid rafts at the cell surface, or forms freely diffusible active multimers with its hydrophobic lipid-modified N- and C-termini buried inside. Ref.2

Tissue specificity: Expressed in the node, notochord, floor plate, and posterior limb bud mesenchyme.

Domain: The sonic hedgehog protein N-product binds calcium and zinc ions; this stabilizes the protein fold and is essential for protein-protein interactions mediated by this domain

By similarity.

Post-translational modification: The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity.Cholesterylation is required for N-product targeting to lipid rafts and multimerization

By similarity.N-palmitoylation of Cys-25 by HHAT is required for N-product multimerization and full activity

By similarity.

Sequence similarities: Belongs to the hedgehog family.