Rat Presenilin 1 (PSEN1) RTU ELISA Kit | PSEN1 rtu elisa kit

Rat Presenilin 1 (PSEN1) ELISA Kit

Cat. Num. AAA4502067

• Reactivity: Rat
• Synonyms: Presenilin 1 (PSEN1); Rat Presenilin 1 (PSEN1) ELISA Kit; FAD; AD3; PS1; PSNL1; S182; Alzheimer Disease 3; Protein S182; Presenilin-1 CTF12; PSEN1 rtu elisa kit

• Reactivity: Rat
• Sequence Length: 468

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.061ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PSEN1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PSEN1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PSEN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PSEN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PSEN1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 47575859
• NCBI GeneID: 29192
• NCBI Accession #: NP_062036.2
• NCBI GenBank Nucleotide #: NM_019163.3
• UniProt Accession #: P97887; P97529
• Molecular Weight: 52,790 Da
• NCBI Official Full Name: presenilin-1
• NCBI Official Synonym Full Names: presenilin 1
• NCBI Official Symbol: Psen1
• NCBI Protein Information: presenilin-1
• UniProt Protein Name: Presenilin-1
• Protein Family: Presenilin
• UniProt Gene Name: Psen1
• UniProt Synonym Gene Names: Psnl1; PS-1; PS1-CTF12

NCBI Description

multitransmembrane protein; human homolog mutated in most cases of early onset familial Alzheimer's disease; may mediate retinal lamination and pattern formation [RGD, Feb 2006]

Uniprot Description

Catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein). Requires the presence of the other members of the gamma-secretase complex for protease activity. Plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels. Stimulates cell-cell adhesion via its interaction with CDH1; this stabilizes the complexes between CDH1 (E-cadherin) and its interaction partners CTNNB1 (beta-catenin), CTNND1 and JUP (gamma-catenin). Under conditions of apoptosis or calcium influx, cleaves CDH1. This promotes the disassembly of the complexes between CDH1 and CTNND1, JUP and CTNNB1, increases the pool of cytoplasmic CTNNB1, and thereby negatively regulates Wnt signaling (). Required for normal embryonic brain and skeleton development, and for normal angiogenesis ().