Phosphoglycerate Mutase 1, Brain (PGAM1) ELISA Kit | PGAM1 elisa kit

Rat Phosphoglycerate Mutase 1, Brain (PGAM1) ELISA Kit

Cat. Num. AAA2705514

• Gene Names: Pgam1; Pgmut; PGAM-B
• Reactivity: Rat
• Synonyms: Phosphoglycerate Mutase 1; Brain (PGAM1); Rat Phosphoglycerate Mutase 1; Brain (PGAM1) ELISA Kit; PGAMA; PGAM-B; BPG-dependent PGAM 1; Phosphoglycerate mutase isozyme B; PGAM1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Phosphoglycerate Mutase 1, Brain (PGAM1).; No significant cross-reactivity or interference between Phosphoglycerate Mutase 1, Brain (PGAM1) and analogues was observed.
• Sequence Length: 254

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.118ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phosphoglycerate Mutase 1, Brain (PGAM1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phosphoglycerate Mutase 1, Brain (PGAM1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PGAM1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphoglycerate Mutase 1, Brain (PGAM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphoglycerate Mutase 1, Brain (PGAM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphoglycerate Mutase 1, Brain (PGAM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphoglycerate Mutase 1, Brain (PGAM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 924183757
• NCBI GeneID: 24642
• NCBI Accession #: NP_445742.2
• NCBI GenBank Nucleotide #: NM_053290.2
• UniProt Accession #: P25113; Q6P0K6
• Molecular Weight: 28,832 Da
• NCBI Official Full Name: phosphoglycerate mutase 1
• NCBI Official Synonym Full Names: phosphoglycerate mutase 1
• NCBI Official Symbol: Pgam1
• NCBI Official Synonym Symbols: Pgmut; PGAM-B
• NCBI Protein Information: phosphoglycerate mutase 1
• UniProt Protein Name: Phosphoglycerate mutase 1
• Protein Family: Phosphoglycerate mutase
• UniProt Gene Name: Pgam1
• UniProt Synonym Gene Names: PGAM-B

NCBI Description

catalyzes the interconversion of 3-phosphoglycerate and 2-phosphoglycerate [RGD, Feb 2006]

Uniprot Description

PGAM1: Interconversion of 3- and 2-phosphoglycerate with 2,3- bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity. Homodimer. In mammalian tissues there are two types of phosphoglycerate mutase isozymes: type-M in muscles and type-B in other tissues. Belongs to the phosphoglycerate mutase family. BPG- dependent PGAM subfamily.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 3.1.3.13; EC 5.4.2.11; EC 5.4.2.4; Isomerase; Phosphatase (non-protein)

Chromosomal Location of Human Ortholog: 1q54

Cellular Component: cytoplasm; cytosol; membrane; myelin sheath; nucleus

Molecular Function: 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase activity; bisphosphoglycerate mutase activity; hydrolase activity; phosphoglycerate mutase activity; protein kinase binding

Biological Process: gluconeogenesis; glycolytic process; regulation of glycolysis; regulation of pentose-phosphate shunt; respiratory burst