Human PGAM1 (Phosphoglycerate Mutase 1) CLIA Kit | PGAM1 clia kit

Human PGAM1 (Phosphoglycerate Mutase 1) CLIA Kit

Cat. Num. AAA2532277

• Gene Names: PGAM1; PGAMA; PGAM-B; HEL-S-35
• Reactivity: Human
• Synonyms: PGAM1 (Phosphoglycerate Mutase 1); Human PGAM1 (Phosphoglycerate Mutase 1) CLIA Kit; PGAM1 clia kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinant PGAM1. No significant cross-reactivity or interference between PGAM1 and analogues was observed.
• Sequence Length: 254

• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PGAM1 clia kit

Intended Uses: This CLIA kit applies to the in vitro quantitative determination of PGAM1 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to PGAM1. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PGAM1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PGAM1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of PGAM1. You can calculate the concentration of PGAM1 in the samples by comparing the RLU value of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4505753
• NCBI GeneID: 5223
• NCBI Accession #: NP_002620.1
• NCBI GenBank Nucleotide #: NM_002629.2
• Molecular Weight: 28,804 Da
• NCBI Official Full Name: phosphoglycerate mutase 1
• NCBI Official Synonym Full Names: phosphoglycerate mutase 1 (brain)
• NCBI Official Symbol: PGAM1
• NCBI Official Synonym Symbols: PGAMA; PGAM-B; HEL-S-35
• NCBI Protein Information: phosphoglycerate mutase 1
• UniProt Protein Name: Phosphoglycerate mutase 1
• Protein Family: Phosphoglycerate mutase
• UniProt Gene Name: PGAM1
• UniProt Synonym Gene Names: PGAMA; PGAM-B
• UniProt Entry Name: PGAM1_HUMAN

NCBI Description

Phosphoglyceric acid mutase (EC 2.7.5.3) is widely distributed in mammalian tissues where it catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway (summary by Chen et al., 1974 [PubMed 4811757]).[supplied by OMIM, Nov 2010]

Uniprot Description

PGAM1: Interconversion of 3- and 2-phosphoglycerate with 2,3- bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity. Homodimer. In mammalian tissues there are two types of phosphoglycerate mutase isozymes: type-M in muscles and type-B in other tissues. Belongs to the phosphoglycerate mutase family. BPG- dependent PGAM subfamily.

Protein type: EC 5.4.2.11; Phosphatase (non-protein); EC 3.1.3.13; EC 5.4.2.4; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Isomerase

Chromosomal Location of Human Ortholog: 10q25.3

Cellular Component: membrane; cytoplasm; cytosol

Molecular Function: protein binding; phosphoglycerate mutase activity; bisphosphoglycerate mutase activity; bisphosphoglycerate phosphatase activity; protein kinase binding; 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase activity

Biological Process: respiratory burst; glycolysis; dephosphorylation; regulation of glycolysis; carbohydrate metabolic process; glucose metabolic process; regulation of pentose-phosphate shunt; gluconeogenesis