Typical Testing Data/Standard Curve (for reference only)
Rat Perilipin 3 (PLIN3) ELISA Kit | PLIN3 elisa kit
Rat Perilipin 3 (PLIN3) ELISA Kit
Gene Names
PLIN1; PERI; PLIN; FPLD4
Reactivity
Rat
Synonyms
Perilipin 3 (PLIN3); Rat Perilipin 3 (PLIN3) ELISA Kit; M6PRBP1; TIP47; PP17; Mannose-6-Phosphate Receptor Binding Protein 1; Placental Protein 17; 47 kDa mannose 6-phosphate receptor-binding protein; Cargo selection TIP47; PLIN3 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of PLIN3. No significant cross-reactivity or interference between PLIN3 and analogues was observed.
Sequence Length
522
Application Notes
Enzyme-linked immunosorbent assay for Antigen Detection.
Samples
Rat tissue homogenates, cell lysates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
< 0.055ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PLIN3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PLIN3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PLIN3 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PLIN3 in rat tissue homogenates, cell lysates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to PLIN3. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to PLIN3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PLIN3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PLIN3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to PLIN3. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to PLIN3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PLIN3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PLIN3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for PLIN3 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
55,990 Da
NCBI Official Full Name
perilipin
NCBI Official Synonym Full Names
perilipin 1
NCBI Official Symbol
PLIN1
NCBI Official Synonym Symbols
PERI; PLIN; FPLD4
NCBI Protein Information
perilipin-1
UniProt Protein Name
Perilipin-1
Protein Family
UniProt Gene Name
PLIN1
UniProt Synonym Gene Names
PERI; PLIN
NCBI Description
The protein encoded by this gene coats lipid storage droplets in adipocytes, thereby protecting them until they can be broken down by hormone-sensitive lipase. The encoded protein is the major cAMP-dependent protein kinase substrate in adipocytes and, when unphosphorylated, may play a role in the inhibition of lipolysis. Alternatively spliced transcript variants varying in the 5' UTR, but encoding the same protein, have been found for this gene. [provided by RefSeq, Feb 2009]
Uniprot Description
Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness. Plays a role in unilocular lipid droplet formation by activating CIDEC. Their interaction promotes lipid droplet enlargement and directional net neutral lipid transfer. May modulate lipolysis and triglyceride levels.
Research Articles on PLIN3
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Product Notes
The Rat PLIN3 plin1 (Catalog #AAA2104933) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2104933 ELISA Kit recognizes Rat PLIN3. Enzyme-linked immunosorbent assay for Antigen Detection. Researchers should empirically determine the suitability of the PLIN3 plin1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Perilipin 3 (PLIN3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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