Human Perilipin 1 (PLIN1) ELISA Kit | PLIN1 elisa kit

Human Perilipin 1 (PLIN1) ELISA Kit

Cat. Num. AAA2019054

• Gene Names: PLIN1; PERI; PLIN; FPLD4
• Reactivity: Human
• Synonyms: Perilipin 1 (PLIN1); Human Perilipin 1 (PLIN1) ELISA Kit; PERI; PLIN1; Lipid Droplet Associated Protein; PLIN1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PLIN1. No significant cross-reactivity or interference between PLIN1 and analogues was observed.
• Sequence Length: 522

• Samples: Human tissue homogenates, cell lysates and other biological fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.054ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PLIN1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PLIN1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PLIN1 elisa kit

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to PLIN1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to PLIN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PLIN1, biotin-conjugated antibody and enzymeconjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PLIN1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for PLIN1 elisa kit

Metabolic pathway

NCBI and Uniprot Product Information

• NCBI GI #: 223718203
• NCBI GeneID: 5346
• NCBI Accession #: NP_001138783.1
• NCBI GenBank Nucleotide #: NM_001145311.1
• UniProt Accession #: O60240; Q8N5Y6
• Molecular Weight: 55,990 Da
• NCBI Official Full Name: perilipin-1
• NCBI Official Synonym Full Names: perilipin 1
• NCBI Official Symbol: PLIN1
• NCBI Official Synonym Symbols: PERI; PLIN; FPLD4
• NCBI Protein Information: perilipin-1
• UniProt Protein Name: Perilipin-1
• Protein Family: Perilipin
• UniProt Gene Name: PLIN1
• UniProt Synonym Gene Names: PERI; PLIN
• UniProt Entry Name: PLIN1_HUMAN

NCBI Description

The protein encoded by this gene coats lipid storage droplets in adipocytes, thereby protecting them until they can be broken down by hormone-sensitive lipase. The encoded protein is the major cAMP-dependent protein kinase substrate in adipocytes and, when unphosphorylated, may play a role in the inhibition of lipolysis. Alternatively spliced transcript variants varying in the 5' UTR, but encoding the same protein, have been found for this gene. [provided by RefSeq, Feb 2009]

Uniprot Description

perilipin 1: an adipocyte protein of the peripilin family. A modulator of adipocyte lipid metabolism, coating lipid storage droplets to protect them from being broken down by hormone-sensitive lipase. A major cAMP-dependent protein kinase-substrate in adipocytes, also dephosphorylated by PP1. When phosphorylated, may be maximally sensitive to HSL and when unphosphorylated, may play a role in the inhibition of lipolysis.

Protein type: Lipid-binding

Chromosomal Location of Human Ortholog: 15q26

Cellular Component: endoplasmic reticulum; lipid particle; cytosol

Molecular Function: lipid binding

Biological Process: triacylglycerol catabolic process; lipid metabolic process

Disease: Lipodystrophy, Familial Partial, Type 4