Rat PCSK9 ELISA Kit | PCSK9 elisa kit

Rat PCSK9 PicoKine ELISA Kit

Cat. Num. AAA1751606

• Gene Names: PCSK9; FH3; PC9; NARC1; LDLCQ1; NARC-1; HCHOLA3
• Reactivity: Rat
• Synonyms: PCSK9; Rat PCSK9 PicoKine ELISA Kit; Rat PCSK9; PCSK9 elisa kit

• Reactivity: Rat
• Specificity: Natural and recombinant Rat Ntrk3
• Sequence Length: 692

• Samples: Cell Culture Supernates And Cell Lysates
• Detection Range: 156pg/ml-10000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PCSK9 elisa kit

Principle of the Assay: The Picokine Rat Ntrk3 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Rat Ntrk3 with a 96-well strip plate that is pre-coated with antibody specific for Ntrk3. The detection antibody is a biotinylated antibody specific for Ntrk3. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Rat Ntrk3 with immunogen: Expression system for standard: NSO, Immunogen sequence: C32-T429. The kit is analytically validated with ready to use reagents. To measure Rat Ntrk3, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Rat Ntrk3 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Rat Ntrk3 in the sample!!Background/Introduction: NTRK3 (Neurotrophic Tyrosine Kinase Receptor Type 3), also known as TRKC, is a protein that in humans is encoded by the NTRK3 gene. By PCR analysis of a somatic cell hybrid panel and by fluorescence in situ hybridization with the cDNA clone, it mapped the NTRK3 gene to 15q24-q25. TRKC, a member of the TRK family of tyrosine protein kinase genes. They found that TRKC is preferentially expressed in the brain; in situ hybridization studies showed transcripts in the hippocampus, cerebral cortex, and the granular cell layer of the cerebellum. By functional studies in HeLa cells, 5 miRNAs regulate the truncated form of NTRK3.

NCBI and Uniprot Product Information

• NCBI GI #: 31317307
• NCBI GeneID: 255738
• NCBI Accession #: NP_777596.2
• NCBI GenBank Nucleotide #: NM_174936.3
• UniProt Accession #: Q8NBP7
• NCBI Official Full Name: proprotein convertase subtilisin/kexin type 9 preproprotein
• NCBI Official Synonym Full Names: proprotein convertase subtilisin/kexin type 9
• NCBI Official Symbol: PCSK9
• NCBI Official Synonym Symbols: FH3; PC9; NARC1; LDLCQ1; NARC-1; HCHOLA3
• NCBI Protein Information: proprotein convertase subtilisin/kexin type 9
• Protein Family: Proprotein convertase subtilisin/kexin

NCBI Description

This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an autocatalytic processing event with its prosegment in the ER and is constitutively secreted as an inactive protease into the extracellular matrix and trans-Golgi network. It is expressed in liver, intestine and kidney tissues and escorts specific receptors for lysosomal degradation. It plays a role in cholesterol and fatty acid metabolism. Mutations in this gene have been associated with autosomal dominant familial hypercholesterolemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]