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Western Blot (WB) (Western blot detection of Musashi-1 in U87MG, C6, IMR32, mouse brain and PC12 cell lysates and using Musashi-1 mouse mAb (1:1000 diluted).Predicted band size: 39KDa.Observed band size:39KDa.)

Musashi-1 Monoclonal Antibody | anti-MSI1 antibody

Anti-Musashi-1 Mouse mAb

Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity Purified
Synonyms
Musashi-1; Monoclonal Antibody; Anti-Musashi-1 Mouse mAb; MSI1; Msi 1; Msi1; MSI1H_HUMAN; Musashi homolog 1; Musashi1; RNA binding protein Musashi homolog 1; RNA-binding protein Musashi homolog 1; anti-MSI1 antibody
Ordering
For Research Use Only!
Host
Mouse
Source: Human
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
3F8-E6-B9
Purity/Purification
Affinity Purified
Form/Format
Purified mouse monoclonal in PBS (pH 7.4) containing with 0.2% sodium azide, 50% glycerol, 0.1%BSA
Concentration
1mg/ml (varies by lot)
Sequence Length
362
Applicable Applications for anti-MSI1 antibody
Western Blot (WB)
Application Notes
WB: 1:1000
Immunogen
Purified recombinant human Musashi-1 protein fragments expressed in E Coli.
Sensitivity
This antibody detects endogenous levels of Musashi-1 and does not cross-react with related proteins.
Sequence Similarities
Belongs to the Musashi family.Contains 2 RRM (RNA recognition motif) domains.
Subcellular Location
Cytoplasm, Nucleus

Western Blot (WB)

(Western blot detection of Musashi-1 in U87MG, C6, IMR32, mouse brain and PC12 cell lysates and using Musashi-1 mouse mAb (1:1000 diluted).Predicted band size: 39KDa.Observed band size:39KDa.)

Western Blot (WB) (Western blot detection of Musashi-1 in U87MG, C6, IMR32, mouse brain and PC12 cell lysates and using Musashi-1 mouse mAb (1:1000 diluted).Predicted band size: 39KDa.Observed band size:39KDa.)
Related Product Information for anti-MSI1 antibody
Entrez Summary: This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Expression of this gene has been correlated with the grade of the malignancy and proliferative activity in gliomas and melanomas. A pseudogene for this gene is located on chromosome 11q13.
UniPort Summary Function: RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U1-3A

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
39
NCBI Official Full Name
RNA-binding protein Musashi homolog 1
NCBI Official Synonym Full Names
musashi RNA binding protein 1
NCBI Official Symbol
MSI1
NCBI Protein Information
RNA-binding protein Musashi homolog 1
UniProt Protein Name
RNA-binding protein Musashi homolog 1
Protein Family
UniProt Gene Name
MSI1
UniProt Synonym Gene Names
Musashi-1

NCBI Description

This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Expression of this gene has been correlated with the grade of the malignancy and proliferative activity in gliomas and melanomas. A pseudogene for this gene is located on chromosome 11q13. [provided by RefSeq, Jul 2008]

Uniprot Description

Musashi-1: RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'- GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'- [GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system. Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines. Belongs to the Musashi family.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 12q24.31

Molecular Function: RNA binding

Biological Process: nervous system development

Research Articles on MSI1

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Product Notes

The MSI1 msi1 (Catalog #AAA475018) is an Antibody produced from Mouse Source: Human and is intended for research purposes only. The product is available for immediate purchase. The Anti-Musashi-1 Mouse mAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Musashi-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000. Researchers should empirically determine the suitability of the MSI1 msi1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Musashi-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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