Typical Testing Data/Standard Curve (for reference only)
Bovine Alpha-1-Acid Glycoprotein (a1AGP) ELISA Kit | a1AGP elisa kit
Cattle Alpha-1-Acid Glycoprotein (a1AGP) ELISA Kit
Gene Names
ORM1; ORM; AGP1; AGP-A; HEL-S-153w
Reactivity
Bovine
Synonyms
Alpha-1-Acid Glycoprotein (a1AGP); Cattle Alpha-1-Acid Glycoprotein (a1AGP) ELISA Kit; AGP1; a1-AGP; ORM1; OMD 1; Orosomucoid 1; a1AGP elisa kit
Reactivity
Bovine
Specificity
This assay has high sensitivity and excellent specificity for detection of a1AGP. No significant cross-reactivity or interference between a1AGP and analogues was observed.
Sequence Length
201
Samples
Serum, plasma, urine and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
15.6-1,000ng/mL
Sensitivity
< 7.0ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level a1AGP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level a1AGP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for a1AGP elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of a1AGP in bovine serum, plasma, urine and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to a1AGP. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to a1AGP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain a1AGP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of a1AGP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to a1AGP. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to a1AGP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain a1AGP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of a1AGP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
23,512 Da
NCBI Official Full Name
alpha-1 acid glycoprotein
NCBI Official Synonym Full Names
orosomucoid 1
NCBI Official Symbol
ORM1
NCBI Official Synonym Symbols
ORM; AGP1; AGP-A; HEL-S-153w
NCBI Protein Information
alpha-1-acid glycoprotein 1
UniProt Protein Name
Alpha-1-acid glycoprotein 1
UniProt Gene Name
ORM1
UniProt Synonym Gene Names
AGP1; AGP 1; OMD 1
NCBI Description
This gene encodes a key acute phase plasma protein. Because of its increase due to acute inflammation, this protein is classified as an acute-phase reactant. The specific function of this protein has not yet been determined; however, it may be involved in aspects of immunosuppression. [provided by RefSeq, Jul 2008]
Uniprot Description
Functions as transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain. Also binds synthetic drugs and influences their distribution and availability in the body. Appears to function in modulating the activity of the immune system during the acute-phase reaction.
Research Articles on a1AGP
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Product Notes
The Bovine a1AGP orm1 (Catalog #AAA2702511) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2702511 ELISA Kit recognizes Bovine a1AGP. It is sometimes possible for the material contained within the vial of "Alpha-1-Acid Glycoprotein (a1AGP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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