Rabbit alpha Tubulin 1A Polyclonal Antibody | anti-TUBA1A antibody

alpha Tubulin 1A antibody

Cat. Num. AAA837757

• Applications: Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
• Purity: alpha Tubulin 1A antibody was purified by antigen-affinity chromatography
• Synonyms: alpha Tubulin 1A; Polyclonal Antibody; alpha Tubulin 1A antibody; Polyclonal alpha Tubulin 1A; Anti-alpha Tubulin 1A; alpha Tubulin A 1; hum-a-tub1; LIS3; tubulin B-alpha-1; tubulin alpha 3; B-alpha-1; alpha Tubulin A-1; tubulin alpha-1A chain; TUBA1A; FLJ25113; hum-a-tub2; TUBA3; tubulin alpha brain-specific; anti-TUBA1A antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: alpha Tubulin 1A antibody was purified by antigen-affinity chromatography
• Form/Format: Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
• Concentration: 0.91 mg/ml (varies by lot)
• Sequence Length: 251

• Applicable Applications for anti-TUBA1A antibody: Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IF:1:100-1:500; IHC-P: 1:100-1:500; ICC:1:100-1:500; WB: 1:5000-1:20000
• Biological Significance: Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q.
• Cross-Reactivity: Drosophila, Human, Mouse
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of 30 ug of whole cell lysate (A: NIH-3T3) using a 10 % SDS PAGE gel and alpha Tubulin 1A antibody at a dilution of 1:10000)

Western Blot (WB)

(Western blot analysis of 30 ug of whole cell lysate (A: HeLa; B: Hep G2; C: Molt-4) using a 10 % SDS PAGE gel and alpha Tubulin 1A antibody at a dilution of 1:10000)

Immunofluorescence (IF)

(Immunofluorescence analysis of paraformaldehyde-fixed A431, using Tubulin alpha 1A antibody at 1:200 dilution.)

Immunohistochemistry (IHC)

(Immunohistochemical staining of paraffin-embedded SAS xenograft using alpha Tubulin 1A antibody at a dilution of 1:500)

Related Product Information for anti-TUBA1A antibody

Affinity purified Rabbit polyclonal alpha Tubulin 1A antibody

Product Categories/Family for anti-TUBA1A antibody

Cell Biology; Purified Polyclonal Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 669687871
• Molecular Weight: 50 kDa
• NCBI Official Full Name: alpha tubulin 1a, partial
• UniProt Protein Name: Alpha tubulin 1a
• UniProt Gene Name: TUBA1A
• UniProt Entry Name: A0A076G2U6_MYTED

Uniprot Description

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.