Typical Testing Data/Standard Curve (for reference only)
Parathyroid Hormone Receptor 2 (PTHR2) ELISA Kit | PTHR2 elisa kit
Rat Parathyroid Hormone Receptor 2 (PTHR2) ELISA Kit
Gene Names
Pth2r; Pthr2
Reactivity
Rat
Synonyms
Parathyroid Hormone Receptor 2 (PTHR2); Rat Parathyroid Hormone Receptor 2 (PTHR2) ELISA Kit; PTH2; PTH2R; Parathyroid Hormone 2 Receptor; PTHR2 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of PTHR2. No significant cross-reactivity or interference between PTHR2 and analogues was observed.
Sequence Length
546
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Type
Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
0.04ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PTHR2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PTHR2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PTHR2 elisa kit
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of PTHR2 in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to PTHR2 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled PTHR2 and unlabeled PTHR2 (Standards or samples) with the pre-coated antibody specific to PTHR2. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of PTHR2 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of PTHR2 in the sample.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to PTHR2 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled PTHR2 and unlabeled PTHR2 (Standards or samples) with the pre-coated antibody specific to PTHR2. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of PTHR2 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of PTHR2 in the sample.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
61,804 Da
NCBI Official Full Name
parathyroid hormone 2 receptor
NCBI Official Synonym Full Names
parathyroid hormone 2 receptor
NCBI Official Symbol
Pth2r
NCBI Official Synonym Symbols
Pthr2
NCBI Protein Information
parathyroid hormone 2 receptor
UniProt Protein Name
Parathyroid hormone 2 receptor
UniProt Gene Name
Pth2r
UniProt Synonym Gene Names
Pthr2; PTH2 receptor
NCBI Description
binds parathyroid hormone and induces cAMP accumulation [RGD, Feb 2006]
Uniprot Description
This is a specific receptor for parathyroid hormone. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. PTH2R may be responsible for PTH effects in a number of physiological systems. It may play a significant role in pancreatic function. PTH2R presence in neurons indicates that it may function as a neurotransmitter receptor.
Research Articles on PTHR2
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Product Notes
The Rat PTHR2 pth2r (Catalog #AAA453775) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA453775 ELISA Kit recognizes Rat PTHR2. It is sometimes possible for the material contained within the vial of "Parathyroid Hormone Receptor 2 (PTHR2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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