Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using IFNA1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)
Rabbit anti-Human, Mouse IFNA1 Polyclonal Antibody | anti-IFNA1 antibody
IFNA1 Polyclonal Antibody
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence
Purity
Affinity Purification
Synonyms
IFNA1; Polyclonal Antibody; IFNA1 Polyclonal Antibody; IFN-alpha: IFN-a; IFL; IFN; IFN-ALPHA; IFNA13; IFNA; MGC138207; MGC138505; MGC138507; anti-IFNA1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Concentration
1mg/ml (varies by lot)
Sequence Length
189
Applicable Applications for anti-IFNA1 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
WB: 1:500 - 1:2000
Species
Human
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of human IFNA1
Calculated Molecular Weight
22kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using IFNA1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using IFNA1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)
Immunofluorescence (IF)
(Immunofluorescence analysis of A549 cells using IFNA1 antibody. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of A549 cells using IFNA1 antibody. Blue: DAPI for nuclear staining.)
Related Product Information for anti-IFNA1 antibody
Interferon-alpha1 is a member of the Type I IFN (1) family best known for their antiviral activity. Most nucleated cells produce one or more Type I IFNs in response to viral infection (2). Secreted Type I IFN then induces viral protective responses in neighboring non-infected cells. Type I IFNs also enhance virus-induced apoptosis (3). Other IFNA11 activities include enhancement of dendritic cell maturation and cytotoxic T cell activity (4). IFNA11 binds to the IFNA1R1 and IFNA1R2 heterodimer (1). Intracellular signaling through the Jak/Stat pathway is best characterized (3). However, the PI3K, ERK, and p38 kinase pathways are also involved (5). The antiviral activities of the IFNs have led to their use in treating viral infections (4). Type I IFNs also appear to have an integral role in several autoimmune diseases (6).
Product Categories/Family for anti-IFNA1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
189
NCBI Official Full Name
Interferon alpha-1/13
NCBI Official Synonym Full Names
interferon, alpha 13
NCBI Official Symbol
IFNA13
NCBI Protein Information
interferon alpha-1/13; leIF D; IFN-alpha-1/13; interferon alpha-D
UniProt Protein Name
Interferon alpha-1/13
Protein Family
UniProt Gene Name
IFNA1
UniProt Synonym Gene Names
IFN-alpha-1/13; LeIF D
UniProt Entry Name
IFNA1_HUMAN
Uniprot Description
IFNA1/13: a cytokine that belongs to the alpha/beta interferon family.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 9p22
Cellular Component: extracellular space; extracellular region
Molecular Function: interferon-alpha/beta receptor binding; cytokine activity
Biological Process: B cell proliferation; adaptive immune response; response to exogenous dsRNA; B cell differentiation; cytokine and chemokine mediated signaling pathway; natural killer cell activation during immune response; regulation of MHC class I biosynthetic process; innate immune response; blood coagulation; defense response to virus; humoral immune response; T cell activation during immune response; positive regulation of peptidyl-serine phosphorylation of STAT protein
Research Articles on IFNA1
1. Observational study of gene-disease association and gene-environment interaction. (HuGE Navigator)
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Product Notes
The IFNA1 ifna1 (Catalog #AAA125081) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IFNA1 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IFNA1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). WB: 1:500 - 1:2000. Researchers should empirically determine the suitability of the IFNA1 ifna1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IFNA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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