Human Adseverin (SCIN) ELISA Kit | SCIN elisa kit

Human Adseverin (SCIN) ELISA Kit

Cat. Num. AAA7206431

• Reactivity: Human
• Synonyms: Adseverin (SCIN); Human Adseverin (SCIN) ELISA Kit; SCIN elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-1ug/mL
• Sensitivity: 0.1ug/mL
• Intended Uses: This SCIN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human SCIN. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SCIN elisa kit

Principle of the assay: SCIN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SCIN antibody and an SCIN-HRP conjugate. The assay sample and buffer are incubated together with SCIN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SCIN concentration since SCIN from samples and SCIN-HRP conjugate compete for the anti-SCIN antibody binding site. Since the number of sites is limited, as more sites are occupied by SCIN from the sample, fewer sites are left to bind SCIN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SCIN concentration in each sample is interpolated from this standard curve.

Product Categories/Family for SCIN elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 162951877
• NCBI GeneID: 85477
• NCBI Accession #: NP_001106177.1
• NCBI GenBank Nucleotide #: NM_001112706.2
• Molecular Weight: 52,814 Da
• NCBI Official Full Name: adseverin isoform 1
• NCBI Official Synonym Full Names: scinderin
• NCBI Official Symbol: SCIN
• NCBI Protein Information: adseverin
• UniProt Protein Name: Adseverin
• Protein Family: Adseverin
• UniProt Gene Name: SCIN
• UniProt Synonym Gene Names: KIAA1905
• UniProt Entry Name: ADSV_HUMAN

NCBI Description

SCIN is a Ca(2+)-dependent actin-severing and -capping protein (Zunino et al., 2001 [PubMed 11568009]).[supplied by OMIM, May 2010]

Uniprot Description

SCIN: Ca(2+)-dependent actin filament-severing protein that is presumed to have a regulatory function in exocytosis by affecting the organization of the microfilament network underneath the plasma membrane. In vitro, also has barbed end capping and nucleating activities in the presence of Ca(2+). Regulates chondrocyte proliferation and differentiation. MAP kinases p38 and ERK1/2 mediate the adseverin-induced accelerated differentiation of non-hypertrophic chondrocytes. Belongs to the villin/gelsolin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 7p21.3

Cellular Component: cytoskeleton; protein complex; cytoplasm; cell cortex

Molecular Function: actin filament binding; phosphatidylinositol-4,5-bisphosphate binding; phosphatidylserine binding; calcium ion binding; actin binding; phosphatidylinositol binding

Biological Process: actin filament severing; negative regulation of cell proliferation; actin filament capping; positive regulation of apoptosis; positive regulation of secretion; positive regulation of megakaryocyte differentiation; regulation of chondrocyte differentiation; actin nucleation; calcium ion-dependent exocytosis