Dog selectin P (granule membrane protein 140kDa, antigen CD62) ELISA Kit | SELP elisa kit

Dog P-selectin, SELP ELISA Kit

Cat. Num. AAA929329

• Reactivity: Dog
• Synonyms: selectin P (granule membrane protein 140kDa; antigen CD62); Dog P-selectin; SELP ELISA Kit; Dog P-selectin (SELP) ELISA kit; RP1-86F14.2; CD62; CD62P; FLJ45155; GMP140; GRMP; LECAM3; PADGEM; PSEL; OTTHUMP00000032609; granulocyte membrane protein; leukocyte-endothelial cell adhesion molecule 3; platelet alpha-granule membrane; SELP elisa kit

• Reactivity: Dog
• Specificity: This assay has high sensitivity and excellent specificity for detection of dog SELP. No significant cross-reactivity or interference between dog SELP and analogues was observed.
• Sequence Length: 646

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 12.5 ng/ml-800 ng/ml.
• Sensitivity: The minimum detectable dose of dog SELP is typically less than 3.12 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SELP elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SELP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SELP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SELP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SELP bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 27806409
• NCBI GeneID: 281486
• NCBI Accession #: NP_776608.1
• NCBI GenBank Nucleotide #: NM_174183.2
• UniProt Accession #: P42201
• Molecular Weight: 71,229 Da
• NCBI Official Full Name: P-selectin
• NCBI Official Synonym Full Names: selectin P (granule membrane protein 140kDa, antigen CD62)
• NCBI Official Symbol: SELP
• NCBI Protein Information: P-selectin; LECAM3; PADGEM; GMP-140; granule membrane protein 140; CD62 antigen-like family member P; leukocyte-endothelial cell adhesion molecule 3; platelet activation dependent granule-external membrane protein
• UniProt Protein Name: P-selectin
• Protein Family: P-selectin
• UniProt Gene Name: SELP
• UniProt Synonym Gene Names: GMP-140; LECAM3; PADGEM
• UniProt Entry Name: LYAM3_BOVIN

Uniprot Description

Function: Ca2+-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1