Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Myosin phosphatase Rho-interacting protein (MPRIP) Standard Curve.)
Rat Myosin Phosphatase Rho-Interacting Protein (MPRIP) ELISA Kit | MPRIP elisa kit
Rat Myosin Phosphatase Rho-Interacting Protein (MPRIP) ELISA Kit
Gene Names
Mprip; Mrip; M-rip; Rhoip3; p116Rip
Reactivity
Rat
Synonyms
Myosin Phosphatase Rho-Interacting Protein (MPRIP); Rat Myosin Phosphatase Rho-Interacting Protein (MPRIP) ELISA Kit; MPRIP; KIAA0864; M-RIP; MRIP; RHOIP3; RIP3; p116Rip; Rho interacting protein 3; myosin phosphatase-Rho interacting protein; MPRIP elisa kit
Reactivity
Rat
Specificity
Rat Myosin phosphatase Rho-interacting protein (MPRIP) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MPRIP. No significant cross-reactivity or interference between Rat MPRIP and analogues was observed.
Sequence Length
993
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Myosin phosphatase Rho-interacting protein (MPRIP) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Myosin phosphatase Rho-interacting protein (MPRIP) Standard Curve.)
Related Product Information for MPRIP elisa kit
Description: This Rat Myosin phosphatase Rho-interacting protein (MPRIP) ELISA Kit employs a two-site sandwich ELISA to quantitate MPRIP in samples. An antibody specific for MPRIP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MPRIP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MPRIP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MPRIP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: MPRIP (Myosin Phosphatase Rho Interacting Protein) is a Protein Coding gene. Among its related pathways are HIV Life Cycle and Cytoskeletal Signaling. GO annotations related to this gene include actin binding. An important paralog of this gene is TRIOBP. Targets myosin phosphatase to the actin cytoskeleton. Required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells.
Background: MPRIP (Myosin Phosphatase Rho Interacting Protein) is a Protein Coding gene. Among its related pathways are HIV Life Cycle and Cytoskeletal Signaling. GO annotations related to this gene include actin binding. An important paralog of this gene is TRIOBP. Targets myosin phosphatase to the actin cytoskeleton. Required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
myosin phosphatase Rho-interacting protein isoform 2
NCBI Official Synonym Full Names
myosin phosphatase Rho interacting protein
NCBI Official Symbol
Mprip
NCBI Official Synonym Symbols
Mrip; M-rip; Rhoip3; p116Rip
NCBI Protein Information
myosin phosphatase Rho-interacting protein
UniProt Protein Name
Myosin phosphatase Rho-interacting protein
Protein Family
UniProt Gene Name
Mprip
UniProt Synonym Gene Names
Mrip; Rhoip3; RIP3
UniProt Entry Name
MPRIP_RAT
NCBI Description
mouse homolog binds F-actin and may play a role in F-actin bundling and cytoskeleton organization [RGD, Feb 2006]
Research Articles on MPRIP
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Product Notes
The Rat MPRIP mprip (Catalog #AAA9712241) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712241 ELISA Kit recognizes Rat MPRIP. It is sometimes possible for the material contained within the vial of "Myosin Phosphatase Rho-Interacting Protein (MPRIP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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