Human sICAM-1 ELISA Kit | sICAM-1 elisa kit

Human sICAM-1 ELISA KIT

Cat. Num. AAA9424820

• Gene Names: ICAM1; BB2; CD54; P3.58
• Reactivity: Human
• Synonyms: sICAM-1; Human sICAM-1 ELISA KIT; Human sICAM-1; sICAM-1 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human sICAM-1 Ligand

• Assay Type: Solid Phase Sandwich ELISA
• Samples: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
• Detection Range: 62.5-4000pg/mL
• Sensitivity: 31pg/mL
• Assay Time: 3 hours
• Sample Volume: 20 uL
• Detection Method: Colorimetric
• Preparation and Storage: Store at 4 degree C

Typical Testing Data/Standard Curve (for reference only)

(Representative standard curve for sICAM-1 ELISA. sICAM-1 was diluted in serial two-fold steps in Sample Diluent.)

Related Product Information for sICAM-1 elisa kit

Intercellular Adhesion Molecule 1 (ICAM-1), also known as CD54, is a nearly ubiquitous transmembrane glycoprotein that plays a key role in leukocyte migration and activation (1, 2). Human ICAM-1 contains five Ig-like domains in its extracellular domain (ECD) and associates into non-covalently linked dimers (3, 4). Soluble forms of monomeric and dimeric ICAM-1 (sICAM-1) can be generated via proteolytic cleavage by cathepsin G, elastase, MMP-9, MMP-14/MT1-MMP, and TACE/ADAM17 (5 - 8). In the mouse, alternate splicing generates isoforms that lack particular Ig-like domains and are differentially sensitive to proteolysis (5). Within the ECD, human ICAM-1 shares 53% amino acid sequence identity with mouse and rat ICAM-1.The principal binding partners of ICAM-1 are the leukocyte integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) (9 - 11). The multivalency of dimeric ICAM-1 increases its strength of interaction with LFA-1 (9, 10). ICAM-1 also binds several non-integrin ligands including CD43/sialophorin, fibrinogen, hyaluronan, rhinoviruses, and Plasmodium falciparum-infected erythrocytes (12 - 16). At sites of inflammation, ICAM-1 is upregulated on endothelial and epithelial cells where it mediates the adhesion and paracellular migration of leukocytes expressing activated LFA-1 and Mac-1 (17 - 20). ICAM-1 ligation prolongs antigen presentation by dendritic cells and promotes T cell proliferation and cytokine release (21 - 23). ICAM-1 activation also participates in angiogenesis, wound healing, and bone metabolism (24 - 26).Soluble ICAM-1 has been reported in serum, cerebrospinal fluid, urine, and bronchoalveolar lavage fluid (2, 27 - 31). Elevated levels of sICAM-1 in these fluids are associated with cardiovascular disease, type 2 diabetes, organ transplant dysfunction, oxidant stress, abdominal fat mass, hypertension, liver disease, and certain malignancies (32 - 40). sICAM-1 promotes angiogenesis and serves as an indicator of vascular endothelial cell activation or damage (41, 42). It also functions as an inhibitor of transmembrane ICAM-1 mediated activities such as monocyte adhesion to activated endothelial cells and sensitivity of tumor cells to NK cell-mediated lysis (7, 8).

NCBI and Uniprot Product Information

• NCBI GI #: 1836075
• NCBI GeneID: 3383
• Molecular Weight: 57,825 Da
• NCBI Official Full Name: sICAM-1, partial
• NCBI Official Synonym Full Names: intercellular adhesion molecule 1
• NCBI Official Symbol: ICAM1
• NCBI Official Synonym Symbols: BB2; CD54; P3.58
• NCBI Protein Information: intercellular adhesion molecule 1
• UniProt Protein Name: Intercellular adhesion molecule 1
• UniProt Gene Name: ICAM1
• UniProt Synonym Gene Names: ICAM-1

NCBI Description

This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008]

Uniprot Description

ICAM1: a type I membrane protein of the immunoglobulin superfamily. Is a ligand for the leukocyte adhesion LFA-1 protein (Integrin alpha-L/beta-2) and a Rhinovirus receptor. Typically expressed on endothelial cells and cells of the immune system. ICAM1 binds to integrins of type CD11a / CD18, or CD11b / CD18. Its expression is activated by p53 in an NF-kappaB-independent manner. Induced by TNFalpha in a process that involves IKKbeta.

Protein type: Cell adhesion; Immunoglobulin superfamily; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: cell surface; extracellular space; focal adhesion; integral to plasma membrane; membrane; plasma membrane

Molecular Function: integrin binding; protein binding; receptor activity; transmembrane receptor activity

Biological Process: adhesion to host; cell adhesion; extracellular matrix organization and biogenesis; heterophilic cell adhesion; leukocyte adhesion; leukocyte migration; membrane to membrane docking; positive regulation of cellular extravasation; regulation of immune response; regulation of leukocyte mediated cytotoxicity; T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell; virion attachment, binding of host cell surface receptor

Disease: Malaria, Susceptibility To