Western Blot (WB)
(Western blot analysis of SH-SY5Y cell lysateusing MPRIP antibody.)
Rabbit MPRIP Antibody | anti-MPRIP antibody
MPRIP Antibody
Gene Names
MPRIP; MRIP; RIP3; M-RIP; RHOIP3; p116Rip
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Synonyms
MPRIP; MPRIP Antibody; KIAA0864; M-RIP; RHOIP3; p116Rip; anti-MPRIP antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Isotype
IgG
Purity/Purification
Affinity purification
Sequence Length
1038
Applicable Applications for anti-MPRIP antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:100
IF: 1:20-1:50
IHC: 1:50-1:100
IF: 1:20-1:50
Immunogen
Fusion protein of human MPRIP
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of SH-SY5Y cell lysateusing MPRIP antibody.)
Western Blot (WB)
(Western blot analysis of SH-SY5Y cell lysateusing MPRIP antibody.)
Related Product Information for anti-MPRIP antibody
MPRIP, also named as KIAA0864, MRIP, RHOIP3 and p116Rip, is required for the regulation of the actin cytoskeleton by RhoA and ROCK1. MRPIP targets myosin phosphatase to the actin cytoskeleton. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells. The antibody recognizes the C-term of isoform2 and isoform3 of MPRIP.
Product Categories/Family for anti-MPRIP antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
117kDa
NCBI Official Full Name
myosin phosphatase Rho-interacting protein isoform 1
NCBI Official Synonym Full Names
myosin phosphatase Rho interacting protein
NCBI Official Symbol
MPRIP
NCBI Official Synonym Symbols
MRIP; RIP3; M-RIP; RHOIP3; p116Rip
NCBI Protein Information
myosin phosphatase Rho-interacting protein
UniProt Protein Name
Myosin phosphatase Rho-interacting protein
Protein Family
UniProt Gene Name
MPRIP
UniProt Synonym Gene Names
; M-RIP; RIP3
Uniprot Description
Targets myosin phosphatase to the actin cytoskeleton. Required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells.
Research Articles on MPRIP
Similar Products
Product Notes
The MPRIP mprip (Catalog #AAA8502136) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MPRIP Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MPRIP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:1000 IHC: 1:50-1:100 IF: 1:20-1:50. Researchers should empirically determine the suitability of the MPRIP mprip for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MPRIP, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
