Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Myelin-associated glycoprotein (MAG) Standard Curve.)
Rat Myelin-Associated Glycoprotein (MAG) ELISA Kit | MAG elisa kit
Rat Myelin-Associated Glycoprotein (MAG) ELISA Kit
Gene Names
Mag; 1B236
Reactivity
Rat
Synonyms
Myelin-Associated Glycoprotein (MAG); Rat Myelin-Associated Glycoprotein (MAG) ELISA Kit; MAG; GMA; S-MAG; SIGLEC-4A; SIGLEC4A; myelin-associated glycoprotein; sialic acid-binding immunoglobulin-like lectin 4A; MAG elisa kit
Reactivity
Rat
Specificity
Rat Myelin-associated glycoprotein (MAG) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MAG. No significant cross-reactivity or interference between Rat MAG and analogues was observed.
Sequence Length
626
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Myelin-associated glycoprotein (MAG) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Myelin-associated glycoprotein (MAG) Standard Curve.)
Related Product Information for MAG elisa kit
Description: This Rat Myelin-associated glycoprotein (MAG) ELISA Kit employs a two-site sandwich ELISA to quantitate MAG in samples. An antibody specific for MAG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MAG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Myelin-associated glycoprotein (MAG) is a cell membrane glycoprotein that is a member of the SIGLEC family of proteins and is a functional ligand of the NOGO-66 receptor, NgR MAG is believed to be involved in myelination during nerve regeneration.Myelin-associated glycoprotein (MAG), a membrane protein of 100 kD, is thought to be involved in the process of myelination (Barton et al., 1987). MAG is a sialic acid-binding SIGLEC protein and is a functional ligand for the NOGO receptor (NGR, or RTN4R) (Liu et al., 2002).Spagnol et al. (1989) found that MAG cDNA clones isolated from a human brain cDNA library had an open reading frame of 1,878 nucleotides encoding a peptide of 626 amino acids with a calculated molecular weight of 69.1 kD.
Background: Myelin-associated glycoprotein (MAG) is a cell membrane glycoprotein that is a member of the SIGLEC family of proteins and is a functional ligand of the NOGO-66 receptor, NgR MAG is believed to be involved in myelination during nerve regeneration.Myelin-associated glycoprotein (MAG), a membrane protein of 100 kD, is thought to be involved in the process of myelination (Barton et al., 1987). MAG is a sialic acid-binding SIGLEC protein and is a functional ligand for the NOGO receptor (NGR, or RTN4R) (Liu et al., 2002).Spagnol et al. (1989) found that MAG cDNA clones isolated from a human brain cDNA library had an open reading frame of 1,878 nucleotides encoding a peptide of 626 amino acids with a calculated molecular weight of 69.1 kD.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
myelin-associated glycoprotein
NCBI Official Synonym Full Names
myelin-associated glycoprotein
NCBI Official Symbol
Mag
NCBI Official Synonym Symbols
1B236
NCBI Protein Information
myelin-associated glycoprotein
UniProt Protein Name
Myelin-associated glycoprotein
Protein Family
UniProt Gene Name
Mag
UniProt Synonym Gene Names
Siglec-4a
NCBI Description
a cell adhesion molecule involved in axonal-glial and glial-glial interactions [RGD, Feb 2006]
Uniprot Description
Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha-2,3-linked sialic acid ().
Research Articles on MAG
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Product Notes
The Rat MAG mag (Catalog #AAA9712341) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712341 ELISA Kit recognizes Rat MAG. It is sometimes possible for the material contained within the vial of "Myelin-Associated Glycoprotein (MAG), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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