Rat Myelin Associated Glycoprotein (MAG) RTU ELISA Kit | MAG rtu elisa kit

Rat Myelin Associated Glycoprotein (MAG) ELISA Kit

Cat. Num. AAA4500888

• Gene Names: Mag; 1B236
• Reactivity: Rat
• Synonyms: Myelin Associated Glycoprotein (MAG); Rat Myelin Associated Glycoprotein (MAG) ELISA Kit; GMA; S-MAG; Siglec-4; SIGLEC4A; Sialic Acid Binding Ig-Like Lectin 4A; MAG rtu elisa kit

• Reactivity: Rat
• Sequence Length: 626

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.065ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MAG rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MAG. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MAG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MAG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MAG in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 8393742
• NCBI GeneID: 29409
• NCBI Accession #: NP_058886.1
• NCBI GenBank Nucleotide #: NM_017190.4
• UniProt Accession #: P07722; P02685; P07723
• Molecular Weight: 64,351 Da
• NCBI Official Full Name: myelin-associated glycoprotein
• NCBI Official Synonym Full Names: myelin-associated glycoprotein
• NCBI Official Symbol: Mag
• NCBI Official Synonym Symbols: 1B236
• NCBI Protein Information: myelin-associated glycoprotein
• UniProt Protein Name: Myelin-associated glycoprotein
• Protein Family: Myelin-associated glycoprotein
• UniProt Gene Name: Mag
• UniProt Synonym Gene Names: Siglec-4a

NCBI Description

a cell adhesion molecule involved in axonal-glial and glial-glial interactions [RGD, Feb 2006]

Uniprot Description

Adhesion molecule that mediates interactions between myelinating cells and neurons by binding to neuronal sialic acid-containing gangliosides and to the glycoproteins RTN4R and RTN4RL2 (PubMed:8995428, PubMed:9298990). Not required for initial myelination, but seems to play a role in the maintenance of normal axon myelination. Protects motoneurons against apoptosis, also after injury; protection against apoptosis is probably mediated via interaction with neuronal RTN4R and RTN4RL2. Required to prevent degeneration of myelinated axons in adults; this probably depends on binding to gangliosides on the axon cell membrane (). Negative regulator of neurite outgrowth; in dorsal root ganglion neurons the inhibition is mediated primarily via binding to neuronal RTN4R or RTN4RL2 and to a lesser degree via binding to neuronal gangliosides (PubMed:17640868). In cerebellar granule cells the inhibition is mediated primarily via binding to neuronal gangliosides (PubMed:17640868). In sensory neurons, inhibition of neurite extension depends only partially on RTN4R, RTN4RL2 and gangliosides (PubMed:19367338). Inhibits axon longitudinal growth (PubMed:9298990). Inhibits axon outgrowth by binding to RTN4R (). Preferentially binds to alpha-2,3-linked sialic acid (PubMed:8995428). Binds ganglioside Gt1b (PubMed:8995428).