Porcine MMP14 (Matrix metalloproteinase-14) ELISA Kit | MMP14 elisa kit

Porcine MMP14 (Matrix metalloproteinase-14) ELISA Kit

Cat. Num. AAA7607278

• Gene Names: MMP14; MMP-14; MMP-X1; MT-MMP; MT1MMP; MTMMP1; WNCHRS; MT1-MMP; MT-MMP 1
• Reactivity: Porcine
• Synonyms: MMP14 (Matrix metalloproteinase-14); Porcine MMP14 (Matrix metalloproteinase-14) ELISA Kit; MMP14 elisa kit

• Reactivity: Porcine
• Specificity: This assay has high sensitivity and excellent specificity for detection of MMP13. No significant cross-reactivity or interference between MMP13 and analogues was observed.
• Sequence Length: 582

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: <0.094ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MMP13 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MMP13 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MMP14 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-MMP13 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-MMP13 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MMP13 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MMP13 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4826834
• NCBI GeneID: 4323
• NCBI Accession #: NP_004986.1
• NCBI GenBank Nucleotide #: NM_004995.4
• UniProt Accession #: P50281; Q6GSF3; Q92678; A8K5L0
• Molecular Weight: 65,894 Da
• NCBI Official Full Name: matrix metalloproteinase-14 preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 14
• NCBI Official Symbol: MMP14
• NCBI Official Synonym Symbols: MMP-14; MMP-X1; MT-MMP; MT1MMP; MTMMP1; WNCHRS; MT1-MMP; MT-MMP 1
• NCBI Protein Information: matrix metalloproteinase-14
• UniProt Protein Name: Matrix metalloproteinase-14
• Protein Family: Matrix metalloproteinase
• UniProt Gene Name: MMP14
• UniProt Synonym Gene Names: MMP-14; MT-MMP 1; MTMMP1; MT1-MMP; MT1MMP

NCBI Description

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion. [provided by RefSeq, Jul 2008]

Uniprot Description

Endopeptidase that degrades various components of the extracellular matrix such as collagen. Activates progelatinase A. Essential for pericellular collagenolysis and modeling of skeletal and extraskeletal connective tissues during development (). May be involved in actin cytoskeleton reorganization by cleaving PTK7 (PubMed:20837484). Acts as a positive regulator of cell growth and migration via activation of MMP15. Involved in the formation of the fibrovascular tissues in association with pro-MMP2 (PubMed:12714657). Cleaves ADGRB1 to release vasculostatin-40 which inhibits angiogenesis (PubMed:22330140).