Rat Liver X Receptor Alpha (LXRa) ELISA Kit | LXRa elisa kit

Rat Liver X Receptor Alpha (LXRa) ELISA Kit

Cat. Num. AAA457075

• Gene Names: Nr1h3; LXRalpha
• Reactivity: Rat
• Synonyms: Liver X Receptor Alpha (LXRa); Rat Liver X Receptor Alpha (LXRa) ELISA Kit; NR1-H3; LXR-A; LXRA; RLD-1; Nuclear Receptor Subfamily 1; Group H; Member 3; Oxysterols receptor LXR-alpha; LXRa elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of LXRa. No significant cross-reactivity or interference between LXRa and analogues was observed.
• Sequence Length: 445

• Samples: Serum, plasma, tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: <0.112ng/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LXRa were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LXRa were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LXRa elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of LXRa in rat serum, plasma, tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to LXRa. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to LXRa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LXRa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of LXRa in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 11465384
• NCBI GeneID: 58852
• UniProt Accession #: Q62685
• Molecular Weight: 50,555 Da
• NCBI Official Full Name: Oxysterols receptor LXR-alpha
• NCBI Official Synonym Full Names: nuclear receptor subfamily 1, group H, member 3
• NCBI Official Symbol: Nr1h3
• NCBI Official Synonym Symbols: LXRalpha
• NCBI Protein Information: oxysterols receptor LXR-alpha
• UniProt Protein Name: Oxysterols receptor LXR-alpha
• UniProt Gene Name: Nr1h3
• UniProt Synonym Gene Names: Lxra

NCBI Description

orphan receptor; involved in the stimulation of CYP7A1 transcription [RGD, Feb 2006]

Uniprot Description

Nuclear receptor. Interaction with RXR shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8. Interplays functionally with RORA for the regulation of genes involved in liver metabolism. Exhibits a ligand-dependent transcriptional activation activity ().