Typical Testing Data/Standard Curve (for reference only)
Mouse Oxysterols receptor LXR-alpha ELISA Kit | Nr1h3 elisa kit
Mouse Oxysterols receptor LXR-alpha ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Oxysterols receptor LXR-alpha. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Oxysterols receptor LXR-alpha and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Oxysterols receptor LXR-alpha, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Oxysterols receptor LXR-alpha in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
LXR-alpha: Orphan receptor. Interaction with RXR shifts RXR from its role as a silent DNA-binding partner to an active ligand- binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half- sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8. Belongs to the nuclear hormone receptor family. NR1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Nuclear receptor; Motility/polarity/chemotaxis; DNA-binding
Cellular Component: cytoplasm; nuclear chromatin; nucleus; receptor complex
Molecular Function: DNA binding; ligand-dependent nuclear receptor activity; metal ion binding; protein binding; protein heterodimerization activity; retinoid X receptor binding; sequence-specific DNA binding; steroid hormone receptor activity; sterol response element binding; transcription factor activity; zinc ion binding
Biological Process: apoptotic cell clearance; cellular lipid metabolic process; cholesterol homeostasis; fatty acid biosynthetic process; lipid homeostasis; negative regulation of inflammatory response; negative regulation of lipid transport; negative regulation of macrophage activation; negative regulation of pinocytosis; negative regulation of proteolysis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of cellular protein metabolic process; positive regulation of cholesterol transport; positive regulation of fatty acid biosynthetic process; positive regulation of lipoprotein lipase activity; positive regulation of toll-like receptor 4 signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of transcription, DNA-dependent; response to progesterone stimulus; steroid hormone mediated signaling; sterol homeostasis; transcription, DNA-dependent
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Product Notes
The Mouse Nr1h3 nr1h3 (Catalog #AAA2881512) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2881512 ELISA Kit recognizes Mouse Nr1h3. It is sometimes possible for the material contained within the vial of "Oxysterols receptor LXR-alpha, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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