Human Liver X Receptor Alpha (LXRa) ELISA Kit | LXRa elisa kit

Human Liver X Receptor Alpha (LXRa) ELISA Kit

Cat. Num. AAA2020125

• Gene Names: NR1H3; LXRA; LXR-a; RLD-1
• Reactivity: Human
• Synonyms: Liver X Receptor Alpha (LXRa); Human Liver X Receptor Alpha (LXRa) ELISA Kit; NR1-H3; LXR-A; LXRA; RLD-1; Nuclear Receptor Subfamily 1; Group H; Member 3; Oxysterols receptor LXR-alpha; LXRa elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Liver X Receptor Alpha (LXRa).; No significant cross-reactivity or interference between Liver X Receptor Alpha (LXRa) and analogues was observed.
• Sequence Length: 447

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.056ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Liver X Receptor Alpha (LXRa) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Liver X Receptor Alpha (LXRa) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LXRa elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Liver X Receptor Alpha (LXRa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Liver X Receptor Alpha (LXRa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Liver X Receptor Alpha (LXRa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Liver X Receptor Alpha (LXRa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for LXRa elisa kit

Signal transduction; Metabolic pathway; Infection immunity; Hepatology

NCBI and Uniprot Product Information

• NCBI GI #: 216409704
• NCBI GeneID: 10062
• UniProt Accession #: Q13133; Q8IW13; Q96H87; A8K3J9; D3DQR1
• Molecular Weight: 45,692 Da
• NCBI Official Full Name: liver X receptor alpha
• NCBI Official Synonym Full Names: nuclear receptor subfamily 1, group H, member 3
• NCBI Official Symbol: NR1H3
• NCBI Official Synonym Symbols: LXRA; LXR-a; RLD-1
• NCBI Protein Information: oxysterols receptor LXR-alpha
• UniProt Protein Name: Oxysterols receptor LXR-alpha
• UniProt Gene Name: NR1H3
• UniProt Synonym Gene Names: LXRA
• UniProt Entry Name: NR1H3_HUMAN

NCBI Description

The protein encoded by this gene belongs to the NR1 subfamily of the nuclear receptor superfamily. The NR1 family members are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. This protein is highly expressed in visceral organs, including liver, kidney and intestine. It forms a heterodimer with retinoid X receptor (RXR), and regulates expression of target genes containing retinoid response elements. Studies in mice lacking this gene suggest that it may play an important role in the regulation of cholesterol homeostasis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

LXR-alpha: Orphan receptor. Interaction with RXR shifts RXR from its role as a silent DNA-binding partner to an active ligand- binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half- sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8. Belongs to the nuclear hormone receptor family. NR1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Nuclear receptor; DNA-binding

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: nucleoplasm; nuclear chromatin; nucleus; receptor complex

Molecular Function: ligand-dependent nuclear receptor activity; protein binding; sterol response element binding; DNA binding; zinc ion binding; sequence-specific DNA binding; transcription coactivator activity; cholesterol binding; steroid hormone receptor activity

Biological Process: negative regulation of lipid transport; positive regulation of fatty acid biosynthetic process; transcription initiation from RNA polymerase II promoter; intracellular receptor-mediated signaling pathway; positive regulation of transcription, DNA-dependent; positive regulation of cellular protein metabolic process; positive regulation of toll-like receptor 4 signaling pathway; lipid homeostasis; negative regulation of pinocytosis; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cholesterol transport; regulation of circadian rhythm; cholesterol homeostasis; negative regulation of inflammatory response; positive regulation of lipoprotein lipase activity; gene expression; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; response to progesterone stimulus; sterol homeostasis; negative regulation of macrophage activation; apoptotic cell clearance