Typical Testing Data/Standard Curve (for reference only)
Rat Leucine Aminopeptidase (LAP) ELISA Kit | LAP elisa kit
Rat Leucine Aminopeptidase (LAP) ELISA Kit
Gene Names
LAP3; LAP; PEPS; LAPEP; HEL-S-106
Reactivity
Rat
Synonyms
Leucine Aminopeptidase (LAP); Rat Leucine Aminopeptidase (LAP) ELISA Kit; LAPEP; LAP3; PEPS; Peptidase S; Leucyl Aminopeptidase; Cytosol aminopeptidase; Proline aminopeptidase; Prolyl aminopeptidase; LAP elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Leucine Aminopeptidase (LAP).
No significant cross-reactivity or interference between Leucine Aminopeptidase (LAP) and analogues was observed.
No significant cross-reactivity or interference between Leucine Aminopeptidase (LAP) and analogues was observed.
Sequence Length
488
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue homogenates and Other Biological Fluids
Detection Range
0.781-50ng/mL
Sensitivity
< 0.37ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Leucine Aminopeptidase (LAP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Leucine Aminopeptidase (LAP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for LAP elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leucine Aminopeptidase (LAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Leucine Aminopeptidase (LAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Leucine Aminopeptidase (LAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Leucine Aminopeptidase (LAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for LAP elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
52,771 Da
NCBI Official Full Name
leucine aminopeptidase
NCBI Official Synonym Full Names
leucine aminopeptidase 3
NCBI Official Symbol
LAP3
NCBI Official Synonym Symbols
LAP; PEPS; LAPEP; HEL-S-106
NCBI Protein Information
cytosol aminopeptidase
UniProt Protein Name
Cytosol aminopeptidase
Protein Family
UniProt Gene Name
LAP3
UniProt Synonym Gene Names
LAPEP; PEPS; LAP-3
UniProt Entry Name
AMPL_HUMAN
Uniprot Description
LAP3: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides. Belongs to the peptidase M17 family. 2 isoforms of the human protein are produced by alternative initiation.
Protein type: Other Amino Acids Metabolism - glutathione; Amino Acid Metabolism - arginine and proline; EC 3.4.11.1; Protease; EC 3.4.11.5; Mitochondrial
Chromosomal Location of Human Ortholog: 4p15.32
Cellular Component: nucleoplasm; focal adhesion; mitochondrion; cytoplasm; trans-Golgi network; nucleus
Molecular Function: metalloexopeptidase activity; manganese ion binding; aminopeptidase activity
Biological Process: proteolysis
Research Articles on LAP
Similar Products
Product Notes
The Rat LAP lap3 (Catalog #AAA2021573) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2021573 ELISA Kit recognizes Rat LAP. It is sometimes possible for the material contained within the vial of "Leucine Aminopeptidase (LAP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
