Typical Testing Data/Standard Curve (for reference only)
Canine Leucine aminopepridase ELISA Kit | LAP elisa kit
Canine Leucine aminopepridase ELISA Kit
Gene Names
LAP3; LAP; PEPS; LAPEP; HEL-S-106
Reactivity
Canine
Synonyms
Leucine aminopepridase; Canine Leucine aminopepridase ELISA Kit; LAP elisa kit
Reactivity
Canine
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
0.5-10ng/mL
Sensitivity
0.1ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for LAP elisa kit
For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
INTENDED USE This LAP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine LAP. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY LAP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LAP antibody and an LAP-HRP conjugate. The assay sample and buffer are incubated together with LAP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LAP concentration since LAP from samples and LAP-HRP conjugate compete for the anti-LAP antibody bindingsite. Since the number of sites is limited, as more sites are occupied by LAP from the sample, fewer sites are left to bind LAP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LAPconcentration in each sample is interpolated from this standard curve.
INTENDED USE This LAP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine LAP. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY LAP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LAP antibody and an LAP-HRP conjugate. The assay sample and buffer are incubated together with LAP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LAP concentration since LAP from samples and LAP-HRP conjugate compete for the anti-LAP antibody bindingsite. Since the number of sites is limited, as more sites are occupied by LAP from the sample, fewer sites are left to bind LAP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LAPconcentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
52,771 Da
NCBI Official Full Name
leucine aminopeptidase
NCBI Official Synonym Full Names
leucine aminopeptidase 3
NCBI Official Symbol
LAP3
NCBI Official Synonym Symbols
LAP; PEPS; LAPEP; HEL-S-106
NCBI Protein Information
cytosol aminopeptidase; LAP-3; peptidase S; leucyl aminopeptidase; prolyl aminopeptidase; proline aminopeptidase; epididymis secretory protein Li 106
UniProt Protein Name
Cytosol aminopeptidase
Protein Family
UniProt Gene Name
LAP3
UniProt Synonym Gene Names
LAPEP; PEPS; LAP-3
UniProt Entry Name
AMPL_HUMAN
Uniprot Description
LAP3: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides. Belongs to the peptidase M17 family. 2 isoforms of the human protein are produced by alternative initiation.
Protein type: Other Amino Acids Metabolism - glutathione; Amino Acid Metabolism - arginine and proline; Mitochondrial; EC 3.4.11.1; Protease; EC 3.4.11.5
Chromosomal Location of Human Ortholog: 4p15.32
Cellular Component: nucleoplasm; focal adhesion; mitochondrion; cytoplasm; trans-Golgi network; nucleus
Molecular Function: metalloexopeptidase activity; manganese ion binding; aminopeptidase activity
Biological Process: proteolysis
Research Articles on LAP
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Product Notes
The Canine LAP lap3 (Catalog #AAA741866) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA741866 ELISA Kit recognizes Canine LAP. It is sometimes possible for the material contained within the vial of "Leucine aminopepridase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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