Mouse CD105 Monoclonal Antibody | anti-CD105 antibody

Mouse Monoclonal [clone 8A1] (IgG2b) to Human CD105

Cat. Num. AAA249075

• Gene Names: ENG; END; HHT1; ORW1
• Reactivity: Dog, Human, Monkey, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot
• Purity: Protein A/G Purified
• Synonyms: CD105; Monoclonal Antibody; Mouse Monoclonal [clone 8A1] (IgG2b) to Human CD105; Anti-CD105 Antibody (clone 8A1) IHC-plus; ENG; CD105 antigen; END; HHT1; ORW; ORW1; Osler-Rendu-Weber syndrome 1; Endoglin; Human CD105; anti-CD105 antibody

• Host: Mouse
• Reactivity: Dog, Human, Monkey, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 8A1
• Specificity: Human CD105
• Purity/Purification: Protein A/G Purified
• Form/Format: PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 625

• Applicable Applications for anti-CD105 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: IF (1:100), IHC-P (10 ug/ml), WB (1:200 - 1:4000)
• Target Species: Human
• Immunogen Description: Full length human recombinant protein of human ENg(NP_000109) produced in HEK293T cell.
• Immunogen Type: Recombinant protein
• Immunogen: CD105 antibody was raised against full length human recombinant protein of human ENg(NP_000109) produced in HEK293T cell.
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-CD105 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescent staining of HeLa cells using anti-ENG mouse monoclonal antibody.)

Western Blot (WB)

(Western blot of extracts (35 ug) from 9 different cell lines by using anti-ENG monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).)

Western Blot (WB)

(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ENG (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ENG.)

Related Product Information for anti-CD105 antibody

Endoglin is a homodimeric transmembrane glycoprotein highly expressed by endothelial cells. It is a component of the transforming growth factor beta receptor complex as it binds TGFB1 and TGFB3 with high affinity. Endoglin has been shown to be a useful marker to identify tumor angiogenesis. Mutations in the endoglin gene produce hereditary hemorrhagic telangiectasia.

NCBI and Uniprot Product Information

• NCBI GI #: 4557555
• NCBI GeneID: 2022
• NCBI Accession #: NP_000109.1
• NCBI GenBank Nucleotide #: NM_000118.3
• UniProt Accession #: P17813; Q14248; Q14926; Q5T9C0
• Molecular Weight: 67,542 Da
• NCBI Official Full Name: endoglin isoform 2
• NCBI Official Synonym Full Names: endoglin
• NCBI Official Symbol: ENG
• NCBI Official Synonym Symbols: END; HHT1; ORW1
• NCBI Protein Information: endoglin; CD105 antigen
• UniProt Protein Name: Endoglin
• UniProt Gene Name: ENG
• UniProt Synonym Gene Names: END
• UniProt Entry Name: EGLN_HUMAN

NCBI Description

This gene encodes a homodimeric transmembrane protein which is a major glycoprotein of the vascular endothelium. This protein is a component of the transforming growth factor beta receptor complex and it binds to the beta1 and beta3 peptides with high affinity. Mutations in this gene cause hereditary hemorrhagic telangiectasia, also known as Osler-Rendu-Weber syndrome 1, an autosomal dominant multisystemic vascular dysplasia. This gene may also be involved in preeclampsia and several types of cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2013]

Uniprot Description

ENG: Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. Homodimer that forms an heteromeric complex with the signaling receptors for transforming growth factor-beta: TGFBR1 and/or TGFBR2. It is able to bind TGF-beta 1, and 3 efficiently and TGF-beta 2 less efficiently. Interacts with TCTEX1D4. Interacts with ARRB2. Endoglin is restricted to endothelial cells in all tissues except bone marrow. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, misc.

Chromosomal Location of Human Ortholog: 9q34.11

Cellular Component: nucleoplasm; extracellular space; focal adhesion; cell surface; cytoplasm; receptor complex; external side of plasma membrane

Molecular Function: transforming growth factor beta receptor activity; protein binding; protein homodimerization activity; transmembrane receptor activity; glycosaminoglycan binding; transforming growth factor beta binding; galactose binding; punt binding; activin binding; transforming growth factor beta receptor, cytoplasmic mediator activity

Biological Process: wound healing; positive regulation of collagen biosynthetic process; cell motility involved in cell locomotion; positive regulation of systemic arterial blood pressure; negative regulation of transcription from RNA polymerase II promoter; response to corticosteroid stimulus; BMP signaling pathway; extracellular matrix disassembly; smooth muscle development; venous blood vessel morphogenesis; regulation of transforming growth factor beta receptor signaling pathway; central nervous system vasculogenesis; regulation of transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; negative regulation of protein amino acid autophosphorylation; heart looping; vasculogenesis; chronological cell aging; cell adhesion; negative regulation of cell migration; positive regulation of BMP signaling pathway; regulation of cell adhesion; cell migration; regulation of phosphorylation; negative regulation of nitric-oxide synthase activity; regulation of cell proliferation; patterning of blood vessels; negative regulation of endothelial cell proliferation; response to hypoxia; artery morphogenesis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; negative regulation of transforming growth factor beta receptor signaling pathway

Disease: Telangiectasia, Hereditary Hemorrhagic, Of Rendu, Osler, And Weber