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Typical Testing Data/Standard Curve (for reference only)

Human Leucine Aminopeptidase (LAP) ELISA Kit | LAP elisa kit

Human Leucine Aminopeptidase (LAP) ELISA Kit

Gene Names
LAP3; LAP; PEPS; LAPEP; HEL-S-106
Reactivity
Human
Synonyms
Leucine Aminopeptidase (LAP); Human Leucine Aminopeptidase (LAP) ELISA Kit; LAP elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
No cross-reaction with other factors.
Sequence Length
519
Samples
Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant LAP concentration
Assay Type
Sandwich
Detection Range
10 ng/ml-0.156 ng/ml
Sensitivity
0.05 ng/ml.
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for LAP elisa kit
Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human LAP monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
52,771 Da
NCBI Official Full Name
leucine aminopeptidase
NCBI Official Synonym Full Names
leucine aminopeptidase 3
NCBI Official Symbol
LAP3
NCBI Official Synonym Symbols
LAP; PEPS; LAPEP; HEL-S-106
NCBI Protein Information
cytosol aminopeptidase; LAP-3; peptidase S; leucyl aminopeptidase; prolyl aminopeptidase; proline aminopeptidase; epididymis secretory protein Li 106
UniProt Protein Name
Cytosol aminopeptidase
Protein Family
UniProt Gene Name
LAP3
UniProt Synonym Gene Names
LAPEP; PEPS; LAP-3
UniProt Entry Name
AMPL_HUMAN

Uniprot Description

LAP3: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides. Belongs to the peptidase M17 family. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: Other Amino Acids Metabolism - glutathione; EC 3.4.11.5; Amino Acid Metabolism - arginine and proline; EC 3.4.11.1; Protease; Mitochondrial

Chromosomal Location of Human Ortholog: 4p15.32

Cellular Component: nucleoplasm; focal adhesion; mitochondrion; cytoplasm; trans-Golgi network; nucleus

Molecular Function: metalloexopeptidase activity; manganese ion binding; aminopeptidase activity

Biological Process: proteolysis

Research Articles on LAP

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Product Notes

The Human LAP lap3 (Catalog #AAA260896) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA260896 ELISA Kit recognizes Human LAP. It is sometimes possible for the material contained within the vial of "Leucine Aminopeptidase (LAP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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