Rat Janus Kinase 3 (JAK3) ELISA Kit | JAK3 elisa kit

Rat Janus Kinase 3 (JAK3) ELISA Kit

Cat. Num. AAA451110

• Gene Names: Jak3; RATJAK3
• Reactivity: Rat
• Synonyms: Janus Kinase 3 (JAK3); Rat Janus Kinase 3 (JAK3) ELISA Kit; JAKL; L-JAK; LJAK; A Protein Tyrosine Kinase; Leukocyte; Leukocyte janus kinase; Tyrosine-protein kinase JAK3; JAK3 elisa kit

• Reactivity: Rat
• Sequence Length: 1100

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 15.625-1000pg/mL
• Sensitivity: 6.2pg/mL
• Intra Assay: CV<10%
• Inter Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 2499671
• NCBI GeneID: 25326
• UniProt Accession #: Q63272
• Molecular Weight: 122,561 Da
• NCBI Official Full Name: Tyrosine-protein kinase JAK3
• NCBI Official Synonym Full Names: Janus kinase 3
• NCBI Official Symbol: Jak3
• NCBI Official Synonym Symbols: RATJAK3
• NCBI Protein Information: tyrosine-protein kinase JAK3
• UniProt Protein Name: Tyrosine-protein kinase JAK3
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: Jak3
• UniProt Synonym Gene Names: JAK-3

NCBI Description

tyrosine kinase; member of the JAK family of protein kinases [RGD, Feb 2006]

Uniprot Description

Non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation. Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their cytoplasmic domain. Then, STAT5A AND STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3. Once activated, dimerized STAT5 translocates to the nucleus and promotes the transcription of specific target genes in a cytokine-specific fashion ().