Rat Janus Kinase 3 (JAK3) ELISA Kit | JAK3 elisa kit

Rat Janus Kinase 3 (JAK3) ELISA Kit

Cat. Num. AAA2705824

• Gene Names: JAK3; JAKL; LJAK; JAK-3; L-JAK; JAK3_HUMAN
• Reactivity: Rat
• Synonyms: Janus Kinase 3 (JAK3); Rat Janus Kinase 3 (JAK3) ELISA Kit; JAKL; L-JAK; LJAK; A Protein Tyrosine Kinase; Leukocyte; Leukocyte janus kinase; Tyrosine-protein kinase JAK3; JAK3 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Janus Kinase 3 (JAK3).; No significant cross-reactivity or interference between Janus Kinase 3 (JAK3) and analogues was observed.
• Sequence Length: 1124

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 15.62-1000pg/mL
• Sensitivity: < 6.2pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Janus Kinase 3 (JAK3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Janus Kinase 3 (JAK3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for JAK3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Janus Kinase 3 (JAK3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Janus Kinase 3 (JAK3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Janus Kinase 3 (JAK3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Janus Kinase 3 (JAK3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4558771
• NCBI GeneID: 3718
• Molecular Weight: 68,279 Da
• NCBI Official Full Name: JAK3_HUMAN
• NCBI Official Synonym Full Names: Janus kinase 3
• NCBI Official Symbol: JAK3
• NCBI Official Synonym Symbols: JAKL; LJAK; JAK-3; L-JAK; JAK3_HUMAN
• NCBI Protein Information: tyrosine-protein kinase JAK3
• UniProt Protein Name: Tyrosine-protein kinase JAK3
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: JAK3
• UniProt Synonym Gene Names: JAK-3; L-JAK

NCBI Description

The protein encoded by this gene is a member of the Janus kinase (JAK) family of tyrosine kinases involved in cytokine receptor-mediated intracellular signal transduction. It is predominantly expressed in immune cells and transduces a signal in response to its activation via tyrosine phosphorylation by interleukin receptors. Mutations in this gene are associated with autosomal SCID (severe combined immunodeficiency disease). [provided by RefSeq, Jul 2008]

Uniprot Description

JAK3: a non-receptor tyrosine-kinase of the Jak family expressed predominantly in immune cells. Mediates signal transduction via the common gamma-chain of cytokine receptors, including IL-2, -4, -7, -9, and -15. Interacts with members of the STAT (signal transduction and activators of transcription) family. Interacts with STAM2 and SHB. Contains two protein kinase domains. Mutations that abrogate JAK3 function cause an autosomal SCID (severe combined immunodeficiency disease). Inhibitor: R017s for organ transplants. Three differentially spliced isoforms have been described.

Protein type: EC 2.7.10.2; JakA family; Kinase, protein; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); TK group

Chromosomal Location of Human Ortholog: 19p13.11

Cellular Component: cytoskeleton; cytosol; endosome; extrinsic component of cytoplasmic side of plasma membrane

Molecular Function: ATP binding; non-membrane spanning protein tyrosine kinase activity; protein binding; protein phosphatase binding; protein tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity; receptor binding

Biological Process: adaptive immune response; B cell differentiation; cell migration; enzyme linked receptor protein signaling pathway; erythrocyte differentiation; inflammatory response; innate immune response; interleukin-7-mediated signaling pathway; interleukin-9-mediated signaling pathway; MAPK cascade; negative regulation of dendritic cell cytokine production; negative regulation of FasL biosynthetic process; negative regulation of interleukin-10 production; negative regulation of interleukin-12 production; negative regulation of T cell activation; negative regulation of T-helper 1 cell differentiation; peptidyl-tyrosine autophosphorylation; peptidyl-tyrosine phosphorylation; positive regulation of T cell proliferation; protein amino acid phosphorylation; regulation of apoptosis; regulation of molecular function; response to interleukin-9; STAT protein import into nucleus; T cell homeostasis; tyrosine phosphorylation of STAT protein

Disease: Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-negative, B Cell-positive, Nk Cell-negative