Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat IL-17 Standard Curve.)
Rat IL-17 ELISA Kit | IL-17 elisa kit
Rat IL-17 ELISA Kit
Gene Names
Il17a; Il17; IL-17; CTLA-8; IL-17A
Reactivity
Rat
Synonyms
IL-17; Rat IL-17 ELISA Kit; IL 17; IL17A; CTLA8; IL-17A; IL17; cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; cytotoxic T-lymphocyte-associated serine esterase 8; interleukin 17 (cytotoxic T-ly; IL-17 elisa kit
Reactivity
Rat
Specificity
Rat IL-17 ELISA Kit has high sensitivity and excellent specificity for detection of Rat IL-17. No significant cross-reactivity or interference between Rat IL-17 and analogues was observed.
Sequence Length
150
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Range
3.13 pg/mL-200 pg/mL
Detection Method
Colorimetric
Limit of Detection
2 pg/mL
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat IL-17 Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat IL-17 Standard Curve.)
Related Product Information for IL-17 elisa kit
Description: Rat IL-17 ELISA Kit employs a two-site sandwich ELISA to quantitate IL-17 in samples. An antibody specific for IL-17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-17 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-17 is added to the wells. After washing, proprietary Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-17 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: IL-17 is a pro-inflammatory cytokine that is secreted by a subset of activated T cells. It is a disulfide-linked homodimer with both glycosylated and non-glycosylated forms. IL-17A induces stromal cells to produce pro-inflammatory and hematopoietic cytokines, and also enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts.
Background: IL-17 is a pro-inflammatory cytokine that is secreted by a subset of activated T cells. It is a disulfide-linked homodimer with both glycosylated and non-glycosylated forms. IL-17A induces stromal cells to produce pro-inflammatory and hematopoietic cytokines, and also enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Interleukin-17A
NCBI Official Synonym Full Names
interleukin 17A
NCBI Official Symbol
Il17a
NCBI Official Synonym Symbols
Il17; IL-17; CTLA-8; IL-17A
NCBI Protein Information
interleukin-17A
UniProt Protein Name
Interleukin-17A
UniProt Gene Name
Il17a
UniProt Synonym Gene Names
Ctla8; Il17; IL-17; IL-17A; CTLA-8
UniProt Entry Name
IL17_RAT
NCBI Description
cytokine; may be involved in immune system function or to cell death and cell survival [RGD, Feb 2006]
Uniprot Description
Ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines.
Research Articles on IL-17
Similar Products
Product Notes
The Rat IL-17 il17a (Catalog #AAA9711595) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711595 ELISA Kit recognizes Rat IL-17. It is sometimes possible for the material contained within the vial of "IL-17, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
