Mouse CRH ELISA Kit | CRH elisa kit
Mouse CRH (Corticotropin Releasing Hormone) ELISA Kit
Reactivity
Mouse
Synonyms
CRH; Mouse CRH (Corticotropin Releasing Hormone) ELISA Kit; CRH elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse CRH. No significant cross-reactivity or interference between Mouse CRH and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse CRH and all the analogues, therefore, cross reaction may still exist.
Sequence Length
55
Samples
Serum, Plasma, Biological Fluids
Assay Type
Competitive
Detection Range
0.313-20ng/mL
Sensitivity
Min: 0.188ng/mL; Max: 20ng/mL
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CRH elisa kit
Description: The kit is a competitive enzyme immunoassay for in vitro quantitative measurement of CRH in Mouse serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Mouse CRH. During the reaction, Mouse CRH in the sample or standard competes with a fixed amount of Mouse CRH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse CRH. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Mouse CRH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Mouse CRH. During the reaction, Mouse CRH in the sample or standard competes with a fixed amount of Mouse CRH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse CRH. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Mouse CRH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
5,876 Da
NCBI Official Full Name
Crh
UniProt Protein Name
Crh
Protein Family
UniProt Gene Name
crh
UniProt Entry Name
F8FKZ4_PAEMK
