Rabbit GRK1 Polyclonal Antibody | anti-GRK1 antibody

GRK1 (A17) Polyclonal Antibody

Cat. Num. AAA3003562

• Gene Names: GRK1; RK; RHOK; GPRK1
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: GRK1; Polyclonal Antibody; GRK1 (A17) Polyclonal Antibody; Rhodopsin kinase; RK; G protein-coupled receptor kinase 1; RHOK; anti-GRK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: GRK1 (A17) polyclonal antibody detects endogenous levels of GRK1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 563

• Applicable Applications for anti-GRK1 antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to the N-terminal of Human GRK1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of GRK1 (A17) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.)

Related Product Information for anti-GRK1 antibody

Heterotrimeric G protein-mediated signal transduction is a dynamically regulated process with the intensity of signal decreasing over time despite the continued presence of the agonist. This phenomenon, referred to as agonistmediated desensitization, involves phosphorylation of the receptor by two classes of enzymes. The first class is comprised of the second messengerregulated kinases, such as c-AMP dependent protein kinase A and protein kinase C. The second class includes the G protein-coupled receptor kinases (GRKs). At least seven members of the GRK family have been identified. These include rhodopsin kinase (GRK 1alpha and beta); two forms of beta-adrenergic receptor kinase: GRK 2 (betaARK, betaARK1) and GRK 3 (betaARK2); IT-11 (GRK 4); GRK 5; GRK 6; and GRK 7. Phosphorylation of receptors by GRKs appears to be strictly dependent on the receptor being in its agonist-activated state.

NCBI and Uniprot Product Information

• NCBI GI #: 4506529
• NCBI GeneID: 6011
• NCBI Accession #: NP_002920.1
• NCBI GenBank Nucleotide #: NP_002920.1
• UniProt Accession #: Q15835
• Molecular Weight: ~ 63kDa
• NCBI Official Full Name: rhodopsin kinase GRK1
• NCBI Official Synonym Full Names: G protein-coupled receptor kinase 1
• NCBI Official Symbol: GRK1
• NCBI Official Synonym Symbols: RK; RHOK; GPRK1
• NCBI Protein Information: rhodopsin kinase GRK1
• UniProt Protein Name: Rhodopsin kinase
• UniProt Gene Name: GRK1
• UniProt Synonym Gene Names: RHOK; RK
• UniProt Entry Name: RK_HUMAN

NCBI Description

This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates rhodopsin and initiates its deactivation. Defects in GRK1 are known to cause Oguchi disease 2 (also known as stationary night blindness Oguchi type-2). [provided by RefSeq, Jul 2008]

Uniprot Description

GRK1: a Ser/Thr of the GRK family. Phosphorylates and inactivates photon-activated rhodopsin. LOF mutations lead to type 2 Oguchi disease, a form of night blindness. A link between RHOK and retinitis pigmentosa is now thought to be unlikely

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, AGC; EC 2.7.11.14; AGC group; GRK family; GRK subfamily

Chromosomal Location of Human Ortholog: 13q34

Molecular Function: G-protein coupled receptor kinase activity; rhodopsin kinase activity; ATP binding; protein kinase activity

Biological Process: rhodopsin mediated signaling; post-embryonic retina morphogenesis in camera-type eye; phototransduction, visible light; regulation of rhodopsin mediated signaling; visual perception; protein amino acid autophosphorylation; photoreceptor cell morphogenesis; regulation of G-protein coupled receptor protein signaling pathway; positive regulation of phosphorylation; negative regulation of apoptosis

Disease: Oguchi Disease 2