Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Krueppel-like factor 15 (KLF15) Standard Curve.)
Human Krueppel-Like Factor 15 (KLF15) ELISA Kit | KLF15 elisa kit
Human Krueppel-Like Factor 15 (KLF15) ELISA Kit
Gene Names
KLF15; KKLF
Reactivity
Human
Synonyms
Krueppel-Like Factor 15 (KLF15); Human Krueppel-Like Factor 15 (KLF15) ELISA Kit; KLF15; DKFZp779M1320; KKLF; kidney-enriched Kruppel-like factor; KLF15 elisa kit
Reactivity
Human
Specificity
Human Krueppel-like factor 15 (KLF15) ELISA Kit has high sensitivity and excellent specificity for detection of Human KLF15. No significant cross-reactivity or interference between Human KLF15 and analogues was observed.
Sequence Length
416
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Krueppel-like factor 15 (KLF15) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Krueppel-like factor 15 (KLF15) Standard Curve.)
Related Product Information for KLF15 elisa kit
Description: This Human Krueppel-like factor 15 (KLF15) ELISA Kit employs a two-site sandwich ELISA to quantitate KLF15 in samples. An antibody specific for KLF15 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KLF15 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLF15 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLF15 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: KLF15 protein is 84% identical to the rat Klf15 protein, contains 3 zinc finger motifs at its C terminus, N-terminal serine-rich stretches, and a central proline-rich segment. EMSA analysis confirmed that KLF15 and MAZ interact with the GA element of the CLCNKA promoter and showed that KLF15 binds with higher affinity and is a functional competitor of MAZ. Northern blot analysis revealed highest expression of a 2.5-kb KLF15 transcript in liver, followed by heart, skeletal muscle, and kidney. No expression was found in bone marrow or lymphoid tissues. KLF15 was also expressed in cardiac and skeletal muscle interstitial cells and in kidney inner medulla, glomeruli, and cortical interstitium. Immunofluorescence microscopy, however, demonstrated no colocalization of KLF15 with CLCNKA.
Background: KLF15 protein is 84% identical to the rat Klf15 protein, contains 3 zinc finger motifs at its C terminus, N-terminal serine-rich stretches, and a central proline-rich segment. EMSA analysis confirmed that KLF15 and MAZ interact with the GA element of the CLCNKA promoter and showed that KLF15 binds with higher affinity and is a functional competitor of MAZ. Northern blot analysis revealed highest expression of a 2.5-kb KLF15 transcript in liver, followed by heart, skeletal muscle, and kidney. No expression was found in bone marrow or lymphoid tissues. KLF15 was also expressed in cardiac and skeletal muscle interstitial cells and in kidney inner medulla, glomeruli, and cortical interstitium. Immunofluorescence microscopy, however, demonstrated no colocalization of KLF15 with CLCNKA.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
Krueppel-like factor 15
NCBI Official Synonym Full Names
Kruppel like factor 15
NCBI Official Symbol
KLF15
NCBI Official Synonym Symbols
KKLF
NCBI Protein Information
Krueppel-like factor 15
UniProt Protein Name
Krueppel-like factor 15
Protein Family
UniProt Gene Name
KLF15
UniProt Synonym Gene Names
KKLF
UniProt Entry Name
KLF15_HUMAN
Uniprot Description
KLF15: Transcriptional activator. Binds to the GA element of the CLCNKA promoter. Belongs to the Sp1 C2H2-type zinc-finger protein family.
Protein type: Transcription factor; C2H2-type zinc finger protein; DNA-binding
Chromosomal Location of Human Ortholog: 3q21.3
Cellular Component: nucleus
Molecular Function: protein binding; metal ion binding
Biological Process: cardiac muscle hypertrophy; glial cell differentiation; transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; glucose transport
Research Articles on KLF15
Similar Products
Product Notes
The Human KLF15 klf15 (Catalog #AAA9715570) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715570 ELISA Kit recognizes Human KLF15. It is sometimes possible for the material contained within the vial of "Krueppel-Like Factor 15 (KLF15), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
