Bovine Glutamate Cysteine Ligase, Modifier Subunit ELISA Kit | GCLM elisa kit

Bovine Glutamate Cysteine Ligase, Modifier Subunit ELISA Kit

Cat. Num. AAA753380

• Gene Names: GCLM; GLCLR
• Reactivity: Bovine
• Synonyms: Glutamate Cysteine Ligase; Modifier Subunit; Bovine Glutamate Cysteine Ligase; Modifier Subunit ELISA Kit; GCLM elisa kit

• Reactivity: Bovine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GCLM elisa kit

Principle of the assay: GCLM ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GCLM antibody and an GCLM-HRP conjugate. The assay sample and buffer are incubated together with GCLM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GCLM concentration since GCLM from samples and GCLM-HRP conjugate compete for the anti-GCLM antibody binding site. Since the number of sites is limited, as more sites are occupied by GCLM from the sample, fewer sites are left to bind GCLM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GCLM concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 27469699
• NCBI GeneID: 2730
• Molecular Weight: 30,727 Da
• NCBI Official Full Name: Glutamate-cysteine ligase, modifier subunit
• NCBI Official Synonym Full Names: glutamate-cysteine ligase, modifier subunit
• NCBI Official Symbol: GCLM
• NCBI Official Synonym Symbols: GLCLR
• NCBI Protein Information: glutamate--cysteine ligase regulatory subunit; GCS light chain; GSC light chain; gamma-ECS regulatory subunit; glutamate--cysteine ligase modifier subunit; glutamate-cysteine ligase regulatory protein; gamma-glutamylcysteine synthetase regulatory subunit; glutamate-cysteine ligase modifier subunit delta2 alternative splicing; glutamate-cysteine ligase (gamma-glutamylcysteine synthetase), regulatory (30.8kD)
• UniProt Protein Name: Glutamate--cysteine ligase regulatory subunit
• Protein Family: Glutamate--cysteine ligase regulatory
• UniProt Gene Name: GCLM
• UniProt Synonym Gene Names: GLCLR
• UniProt Entry Name: GSH0_HUMAN

NCBI Description

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. [provided by RefSeq, Jul 2008]

Uniprot Description

GCLM: Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. [provided by RefSeq, Jul 2008]

Protein type: Other Amino Acids Metabolism - glutathione

Chromosomal Location of Human Ortholog: 1p22.1

Cellular Component: glutamate-cysteine ligase complex; cytosol

Molecular Function: protein heterodimerization activity; glutamate-cysteine ligase activity; enzyme regulator activity; glutamate-cysteine ligase catalytic subunit binding

Biological Process: response to drug; glutamate metabolic process; response to nitrosative stress; cysteine metabolic process; apoptotic mitochondrial changes; sulfur amino acid metabolic process; xenobiotic metabolic process; glutathione biosynthetic process; negative regulation of neuron apoptosis; response to oxidative stress; regulation of mitochondrial depolarization; regulation of blood vessel size; positive regulation of glutamate-cysteine ligase activity

Disease: Myocardial Infarction, Susceptibility To