Rat Glucagon Like Peptide 1 Receptor (GLP1R) ELISA Kit | GLP1R elisa kit

Rat Glucagon Like Peptide 1 Receptor (GLP1R) ELISA Kit

Cat. Num. AAA2705130

• Gene Names: GLP1R; GLP-1; GLP-1R; GLP-1-R
• Reactivity: Rat
• Synonyms: Glucagon Like Peptide 1 Receptor (GLP1R); Rat Glucagon Like Peptide 1 Receptor (GLP1R) ELISA Kit; GLP1R elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Glucagon Like Peptide 1 Receptor (GLP1R).; No significant cross-reactivity or interference between Glucagon Like Peptide 1 Receptor (GLP1R) and analogues was observed.
• Sequence Length: 463

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.057ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glucagon Like Peptide 1 Receptor (GLP1R) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glucagon Like Peptide 1 Receptor (GLP1R) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GLP1R elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucagon Like Peptide 1 Receptor (GLP1R). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glucagon Like Peptide 1 Receptor (GLP1R). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucagon Like Peptide 1 Receptor (GLP1R), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glucagon Like Peptide 1 Receptor (GLP1R) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 166795283
• NCBI GeneID: 2740
• NCBI Accession #: NP_002053.3
• NCBI GenBank Nucleotide #: NM_002062.4
• UniProt Accession #: P43220; Q2M229; Q99669
• Molecular Weight: 53,026 Da
• NCBI Official Full Name: glucagon-like peptide 1 receptor
• NCBI Official Synonym Full Names: glucagon like peptide 1 receptor
• NCBI Official Symbol: GLP1R
• NCBI Official Synonym Symbols: GLP-1; GLP-1R; GLP-1-R
• NCBI Protein Information: glucagon-like peptide 1 receptor
• UniProt Protein Name: Glucagon-like peptide 1 receptor
• Protein Family: Glucagon-like peptide 1 receptor
• UniProt Gene Name: GLP1R
• UniProt Synonym Gene Names: GLP-1 receptor; GLP-1-R; GLP-1R

NCBI Description

This gene encodes a 7-transmembrane protein that functions as a receptor for glucagon-like peptide 1 (GLP-1) hormone, which stimulates glucose-induced insulin secretion. This receptor, which functions at the cell surface, becomes internalized in response to GLP-1 and GLP-1 analogs, and it plays an important role in the signaling cascades leading to insulin secretion. It also displays neuroprotective effects in animal models. Polymorphisms in this gene are associated with diabetes. The protein is an important drug target for the treatment of type 2 diabetes and stroke. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Apr 2016]

Uniprot Description

GLP1R: This is a receptor for glucagon-like peptide 1. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Belongs to the G-protein coupled receptor 2 family.

Protein type: GPCR, family 2; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 6p21.2

Cellular Component: integral component of membrane; integral component of plasma membrane; intracellular; plasma membrane

Molecular Function: glucagon receptor activity; glucagon-like peptide 1 receptor activity; protein binding; transmembrane receptor activity

Biological Process: adenylate cyclase activation; adenylate cyclase-activating G-protein coupled receptor signaling pathway; cAMP-mediated signaling; cell surface receptor signaling pathway; cellular response to glucagon stimulus; elevation of cytosolic calcium ion concentration; G-protein coupled receptor signaling pathway; learning and/or memory; positive regulation of blood pressure; regulation of heart contraction; regulation of insulin secretion; response to stress