Mouse Forkhead Box Protein O1 ELISA Kit | FOXO1 elisa kit

Mouse Forkhead Box Protein O1 ELISA Kit

Cat. Num. AAA763067

• Gene Names: FOXO1; FKH1; FKHR; FOXO1A
• Reactivity: Mouse
• Synonyms: Forkhead Box Protein O1; Mouse Forkhead Box Protein O1 ELISA Kit; FOXO1/FKHR/FOXO1A/Forkhead box protein O1A/Forkhead in rhabdomyosarcoma/FKH1/forkhead box O1/forkhead homolog in rhabdomyosarcoma; FOXO1 elisa kit

• Reactivity: Mouse
• Sequence Length: 655

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for FOXO1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse FOXO1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse FOXO1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse FOXO1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse FOXO1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 9257222
• NCBI GeneID: 2308
• NCBI Accession #: NP_002006.2
• NCBI GenBank Nucleotide #: NM_002015.3
• Molecular Weight: 69,662 Da
• NCBI Official Full Name: forkhead box protein O1
• NCBI Official Synonym Full Names: forkhead box O1
• NCBI Official Symbol: FOXO1
• NCBI Official Synonym Symbols: FKH1; FKHR; FOXO1A
• NCBI Protein Information: forkhead box protein O1
• UniProt Protein Name: Forkhead box protein O1
• Protein Family: Forkhead box protein
• UniProt Gene Name: FOXO1
• UniProt Synonym Gene Names: FKHR; FOXO1A
• UniProt Entry Name: FOXO1_HUMAN

NCBI Description

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008]

Uniprot Description

FOXO1A: a transcription factor of the forkhead family. A central regulator of metabolism in several cell types. May play an important role in myogenic growth and differentiation, and in the regulation of lipolysis in adipocytes by controlling the expression of adipose triglyceride lipase (ATGL), the rate-limiting lipolytic enzyme. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. Contains 1 fork-head domain.

Protein type: Nuclear receptor co-regulator; Oncoprotein; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 13q14.1

Cellular Component: cytoplasm; cytosol; mitochondrion; nucleoplasm; nucleus

Molecular Function: beta-catenin binding; chromatin binding; protein binding; protein phosphatase 2A binding; sequence-specific DNA binding; ubiquitin protein ligase binding

Biological Process: anatomical structure morphogenesis; apoptosis; autophagy; blood vessel development; cell differentiation; cell glucose homeostasis; cellular response to insulin stimulus; cellular response to starvation; endocrine pancreas development; epidermal growth factor receptor signaling pathway; fat cell differentiation; fibroblast growth factor receptor signaling pathway; innate immune response; insulin receptor signaling pathway; negative regulation of apoptosis; negative regulation of fat cell differentiation; negative regulation of stress-activated MAPK cascade; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; nerve growth factor receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of apoptosis; positive regulation of autophagy; positive regulation of gluconeogenesis; positive regulation of protein catabolic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein amino acid acetylation; response to DNA damage stimulus; thermoregulation; transcription, DNA-dependent

Disease: Rhabdomyosarcoma 2