Typical Testing Data/Standard Curve (for reference only)
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Rat Epiregulin (EREG) ELISA Kit | EREG elisa kit
Rat Epiregulin (EREG) ELISA Kit
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to epiregulin. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to epiregulin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain epiregulin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of epiregulin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a secreted peptide hormone and member of the epidermal growth factor (EGF) family of proteins. The encoded protein is a ligand of the epidermal growth factor receptor (EGFR) and the structurally related erb-b2 receptor tyrosine kinase 4 (ERBB4). The encoded protein may be involved in a wide range of biological processes including inflammation, wound healing, oocyte maturation, and cell proliferation. Additionally, the encoded protein may promote the progression of cancers of various human tissues. [provided by RefSeq, Jul 2015]
Uniprot Description
Epiregulin: Ligand of the EGF receptor/EGFR and ERBB4. May be a mediator of localized cell proliferation. As a mitogen it may stimulate cell proliferation and/or angiogenesis.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 4q13.3
Cellular Component: extracellular space; integral to plasma membrane; extracellular region
Molecular Function: protein binding; growth factor activity; epidermal growth factor receptor binding
Biological Process: response to peptide hormone stimulus; nerve growth factor receptor signaling pathway; wound healing; activation of MAPK activity; positive regulation of interleukin-6 biosynthetic process; positive regulation of smooth muscle cell proliferation; female meiosis; negative regulation of smooth muscle cell differentiation; negative regulation of cell proliferation; primary follicle stage, oogenesis; positive regulation of fibroblast proliferation; cell-cell signaling; positive regulation of epidermal growth factor receptor activity; positive regulation of cell proliferation; positive regulation of innate immune response; ovarian cumulus expansion; angiogenesis; negative regulation of epithelial cell proliferation; epidermal growth factor receptor signaling pathway; positive regulation of cytokine production; anatomical structure morphogenesis; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of mitosis; cytokine and chemokine mediated signaling pathway; positive regulation of cytokine biosynthetic process; luteinizing hormone signaling pathway; keratinocyte proliferation; oocyte maturation; ovulation; keratinocyte differentiation; organ morphogenesis; positive regulation of protein kinase activity; mRNA transcription; innate immune response; negative regulation of transcription, DNA-dependent; positive regulation of phosphorylation; positive regulation of DNA replication
Research Articles on EREG
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Product Notes
The Rat EREG ereg (Catalog #AAA2021738) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2021738 ELISA Kit recognizes Rat EREG. It is sometimes possible for the material contained within the vial of "Epiregulin (EREG), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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