Human epiregulin ELISA Kit | EREG elisa kit

Human epiregulin, EREG ELISA Kit

Cat. Num. AAA904261

• Gene Names: EREG; ER
• Reactivity: Human
• Synonyms: epiregulin; Human epiregulin; EREG ELISA Kit; Human epiregulin (EREG) ELISA kit; ER; ; EREG elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human EREG. No significant cross-reactivity or interference between human EREG and analogues was observed.
• Sequence Length: 169

• Samples: Serum, plasma, cell culture supernates, tissue homogenates.
• Assay Type: Sandwich (Quantitative)
• Detection Range: 15.6 pg/ml-1000 pg/ml.
• Sensitivity: 3.9 pg/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EREG elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EREG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EREG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EREG is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EREG bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4557567
• NCBI GeneID: 2069
• NCBI Accession #: NP_001423.1
• NCBI GenBank Nucleotide #: NM_001432.2
• UniProt Accession #: O14944; Q6FH69; B2RC66
• Molecular Weight: 19,044 Da
• NCBI Official Full Name: proepiregulin preproprotein
• NCBI Official Synonym Full Names: epiregulin
• NCBI Official Symbol: EREG
• NCBI Official Synonym Symbols: ER
• NCBI Protein Information: proepiregulin
• UniProt Protein Name: Proepiregulin
• Protein Family: Proepiregulin
• UniProt Gene Name: EREG
• UniProt Synonym Gene Names: EPR
• UniProt Entry Name: EREG_HUMAN

NCBI Description

Epiregulin is a member of the epidermal growth factor family. Epiregulin can function as a ligand of EGFR (epidermal growth factor receptor), as well as a ligand of most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors. [provided by RefSeq, Jul 2008]

Uniprot Description

Epiregulin: Ligand of the EGF receptor/EGFR and ERBB4. May be a mediator of localized cell proliferation. As a mitogen it may stimulate cell proliferation and/or angiogenesis.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: extracellular space; integral to plasma membrane; extracellular region

Molecular Function: protein binding; growth factor activity; epidermal growth factor receptor binding

Biological Process: response to peptide hormone stimulus; nerve growth factor receptor signaling pathway; wound healing; activation of MAPK activity; positive regulation of interleukin-6 biosynthetic process; positive regulation of smooth muscle cell proliferation; female meiosis; negative regulation of smooth muscle cell differentiation; negative regulation of cell proliferation; primary follicle stage, oogenesis; positive regulation of fibroblast proliferation; positive regulation of epidermal growth factor receptor activity; cell-cell signaling; positive regulation of innate immune response; positive regulation of cell proliferation; ovarian cumulus expansion; angiogenesis; negative regulation of epithelial cell proliferation; positive regulation of cytokine production; epidermal growth factor receptor signaling pathway; anatomical structure morphogenesis; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of mitosis; cytokine and chemokine mediated signaling pathway; positive regulation of cytokine biosynthetic process; keratinocyte proliferation; luteinizing hormone signaling pathway; oocyte maturation; keratinocyte differentiation; ovulation; organ morphogenesis; positive regulation of protein kinase activity; mRNA transcription; innate immune response; negative regulation of transcription, DNA-dependent; positive regulation of DNA replication; positive regulation of phosphorylation