Rat EGF ELISA Kit | EGF elisa kit

EGF (Rat) OmniKine ELISA Kit

Cat. Num. AAA9501929

• Reactivity: Rat
• Synonyms: EGF; EGF (Rat) OmniKine ELISA Kit; Epidermal growth factor; EGF elisa kit

• Reactivity: Rat
• Specificity: The OmniKine Rat EGF ELISA is capable of recognizing both recombinant and naturally produced Rat EGF proteins. The antigens listed below were tested at 50 ng/ml and exhibited less than 1% cross reactivity.; Human: EGF; Murine: EGF; The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.; Human: Artemin, Betacellulin, Epigen, Epiregulin, HB-EGF, Heregulinbeta-1
• Form/Format: 12 x 8-Well Microstrips
• Sequence Length: 1133

• Samples: Plasma, serum, lysate
• Assay Type: Sandwich ELISA
• Detection Range: 8-1000 pg/ml
• Sensitivity: 8-1000 pg/ml
• Detection Method: Colorimetric 450 nm
• Subtype: None
• Preparation and Storage: Store at 4 degree C for 6 months.

Testing Data

(The OmniKine Rat EGF ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat EGF proteins within the range of 8-1000 pg/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 6978797
• NCBI GeneID: 25313
• NCBI Accession #: NP_036974.1
• NCBI GenBank Nucleotide #: NM_012842.1
• UniProt Accession #: P07522; Q63183
• Molecular Weight: 124,126 Da
• NCBI Official Full Name: pro-epidermal growth factor
• NCBI Official Synonym Full Names: epidermal growth factor
• NCBI Official Symbol: Egf
• NCBI Protein Information: pro-epidermal growth factor
• UniProt Protein Name: Pro-epidermal growth factor
• Protein Family: EGF-like domain containing protein
• UniProt Gene Name: Egf
• UniProt Synonym Gene Names: EGF

NCBI Description

binds the epidermal growth factor receptor; may play a role in MAP kinase mediated signaling pathways [RGD, Feb 2006]

Uniprot Description

EGF: EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Defects in EGF are the cause of hypomagnesemia type 4 (HOMG4); also known as renal hypomagnesemia normocalciuric. HOMG4 is a disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion. Clinical features include seizures, mild-to mederate psychomotor retardation, and brisk tendon reflexes. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hormone; Ligand, receptor tyrosine kinase; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q21

Cellular Component: extracellular space; integral component of membrane; intracellular; lysosomal membrane; plasma membrane; receptor complex

Molecular Function: calcium ion binding; epidermal growth factor receptor binding; growth factor activity; protein tyrosine kinase activity; Wnt-activated receptor activity; Wnt-protein binding

Biological Process: activation of MAPK activity; activation of MAPKK activity; angiogenesis; branching morphogenesis of a tube; embryonic retina morphogenesis in camera-type eye; epidermal growth factor receptor signaling pathway; kidney development; negative regulation of secretion; peptidyl-tyrosine phosphorylation; positive regulation of cell proliferation; positive regulation of DNA binding; positive regulation of epidermal growth factor-activated receptor activity; positive regulation of fibroblast proliferation; positive regulation of granule cell precursor proliferation; positive regulation of MAP kinase activity; positive regulation of mitotic nuclear division; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphorylation; positive regulation of receptor internalization; positive regulation of transcription, DNA-templated; regulation of peptidyl-tyrosine phosphorylation; regulation of protein secretion; regulation of protein transport; STAT protein import into nucleus; Wnt receptor signaling pathway through beta-catenin