Rat EAAT3 (Excitatory Amino Acid Transporter 3) ELISA Kit | EAAT3 elisa kit

Rat EAAT3 (Excitatory Amino Acid Transporter 3)ELISA Kit

Cat. Num. AAA7608256

• Gene Names: Slc1a1; Eaac1; Eaat3; REAAC1
• Reactivity: Rat
• Synonyms: EAAT3 (Excitatory Amino Acid Transporter 3); Rat EAAT3 (Excitatory Amino Acid Transporter 3)ELISA Kit; Excitatory amino acid transporter 3; Excitatory amino-acid carrier 1; Sodium-dependent glutamate/aspartate transporter 3; Solute carrier family 1 member 1; Slc1a1; Eaac1; Eaat3; EAAT3 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of EAAT3. No significant cross-reactivity or interference between EAAT3 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for est. ~6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EAAT3 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 1124986
• NCBI GeneID: 25550
• UniProt Accession #: P51907; O88389; Q63727; Q9JJP8
• Molecular Weight: 56,772 Da
• NCBI Official Full Name: glutamate transporter REAAC1
• NCBI Official Synonym Full Names: solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1
• NCBI Official Symbol: Slc1a1
• NCBI Official Synonym Symbols: Eaac1; Eaat3; REAAC1
• NCBI Protein Information: excitatory amino acid transporter 3; excitatory amino acid carrier 1; excitatory amino-acid carrier 1; solute carrier family 1, member 1; excitatory amino acid transporter-3; sodium-dependent glutamate/aspartate transporter 3; Solute carrier family 1 A1 (
• UniProt Protein Name: Excitatory amino acid transporter 3
• UniProt Gene Name: Slc1a1
• UniProt Synonym Gene Names: Eaac1; Eaat3
• UniProt Entry Name: EAA3_RAT

NCBI Description

mediates sodium ion dependent high affinity glutamate transport and proton cotransport; plays a role in maintenance of neuronal function [RGD, Feb 2006]

Uniprot Description

SLC1A1: Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Negatively regulated by ARL6IP5. Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A1 subfamily.

Protein type: Membrane protein, integral; Transporter, SLC family; Membrane protein, multi-pass; Transporter

Cellular Component: apical plasma membrane; integral to membrane; plasma membrane

Molecular Function: protein binding; cysteine transmembrane transporter activity; glutamate binding; sodium:dicarboxylate symporter activity; glutamate:sodium symporter activity; L-glutamate transmembrane transporter activity

Biological Process: L-glutamate import; L-glutamate transport; cysteine transport; protein homooligomerization