STAR recombinant protein

STAR, 64-285aa, Human, His tag E Coli

Cat. Num. AAA204495

• Gene Names: STAR; STARD1
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: STAR; 64-285aa; Human; His tag E Coli; Steroidogenic acute regulatory protein mitochondrial; Steroidogenic acute regulatory protein; mitochondrial; ; STAR recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol, 2mM DTT
• Concentration: 1.0 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MEETLYSDQE LAYLQQGEEA MQKALGILSN QEGWKKESQQ DNGDKVMSKV VPDVGKVFRL EVVVDQPMER LYEELVERME AMGEWNPNVK EIKVLQKIGK DTFITHELAA EAAGNLVGPR DFVSVRCAKR RGSTCVLAGM ATDFGNMPEQ KGVIRAEHGP TCMVLHPLAG SPSKTKLTWL LSIDLKGWLP KSIINQVLSQ TQVDFANHLR KRLESHPASE ARC
• Sequence Length: 285

• Applicable Applications for STAR recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for STAR recombinant protein

STAR, also known as steroidogenic acute regulatory protein, appears to mediate the rapid increase in pregnenolone synthesis stimulated by tropic hormones. It increases pregnenolone synthesis more than four-fold and a major STAR transcript of 1.6 kb is found in ovary and testis. During ongoing growth and differentiation of the follicle of the ovary, the immunoreactivity of it tends to shift from the granulosa cells of early antral follicles to the theca cell layers in the adult. Recombinant human STAR protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.

Product Categories/Family for STAR recombinant protein

Cancer

References

Borthwick F. et al. (2009) FEBS Lett. 583:1147-1153.; Mikhaylova I.V. et al. (2008) J. Endocrinol.199: 435-444.

NCBI and Uniprot Product Information

• NCBI GI #: 56243551
• NCBI GeneID: 6770
• NCBI Accession #: NP_000340
• NCBI GenBank Nucleotide #: NM_000349.2
• UniProt Accession #: P49675; Q16396
• Molecular Weight: 27.1 kDa (243aa), confirmed by MALDI-TOF
• NCBI Official Full Name: steroidogenic acute regulatory protein, mitochondrial
• NCBI Official Synonym Full Names: steroidogenic acute regulatory protein
• NCBI Official Symbol: STAR
• NCBI Official Synonym Symbols: STARD1
• NCBI Protein Information: steroidogenic acute regulatory protein, mitochondrial
• UniProt Protein Name: Steroidogenic acute regulatory protein, mitochondrial
• Protein Family: Starch synthase
• UniProt Gene Name: STAR
• UniProt Synonym Gene Names: STARD1; StAR; StARD1

NCBI Description

The protein encoded by this gene plays a key role in the acute regulation of steroid hormone synthesis by enhancing the conversion of cholesterol into pregnenolone. This protein permits the cleavage of cholesterol into pregnenolone by mediating the transport of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane. Mutations in this gene are a cause of congenital lipoid adrenal hyperplasia (CLAH), also called lipoid CAH. A pseudogene of this gene is located on chromosome 13. [provided by RefSeq, Jul 2008]

Uniprot Description

Plays a key role in steroid hormone synthesis by enhancing the metabolism of cholesterol into pregnenolone. Mediates the transfer of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane where it is cleaved to pregnenolone.